An Online Respiratory Quotient-Feedback Strategy of Feeding Yeast Extract for Efficient Arachidonic Acid Production by Mortierella alpina
文献类型：期刊论文
| 作者 | Li, Xiangyu1,2,4,5; Yu, Chao1,2; Yao, Jianming1,2,4,5 ; Wang, Zhiming1,2,3; Lu, Shuhuan1,2
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| 刊名 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
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| 出版日期 | 2018-01-22 |
| 卷号 | 5页码:11 |
| ISSN号 | 2296-4185 |
| DOI | 10.3389/fbioe.2017.00083 |
| 通讯作者 | Yao, Jianming(jmyao@ipp.ac.cn) ; Wang, Zhiming(jimmy_wang@cabio.cn) |
| 英文摘要 | Mortierella alpina (M. alpina) is well known for arachidonic acid (ARA) production. However, low efficiency and unstableness are long existed problems for industrial production of ARA by M. alpina due to the lack of online regulations. The aim of the present work is to develop an online-regulation strategy for efficient and stable ARA production in industry. The strategy was developed in 50 L fermenters and then applied in a 200 m(3) fermenter. Results indicated that yeast extract (YE) highly increased cell growth in shake flask, it was then used in bioreactor fermentation by various feeding strategies. Feeding YE to control respiratory quotient (RQ) at 1.1 during 0-48 h and at 1.5 during 48-160 h, dry cell weight, and ARA titer reached 53.1 and 11.49 g/L in 50 L fermenter, which were increased by 79.4 and 36.9% as compared to that without YE feeding, respectively. Then, the online RQ-feedback strategy was applied in 200 m(3) bioreactor fermentation and an average ARA titer of 16.82 g/L was obtained from 12 batches, which was 41.0% higher than the control batches. This is the first report on successful application of online RQ-feedback control of YE in ARA production, especially in an industrial scale of 200 m(3) fermentation. It could be applied to other industrial production of microbial oil by oleaginous microorganisms. |
| 资助项目 | Chinese National High Technology Research and Development Program[2014AA021703] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000440249900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program ; Chinese National High Technology Research and Development Program |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/40990]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yao, Jianming; Wang, Zhiming |
| 作者单位 | 1.CABIO Bioengn Wuhan Co Ltd, Wuhan, Hubei, Peoples R China 2.Hubei Prov Nutr Chem Biosynthet Engn Technol Res, Wuhan, Hubei, Peoples R China 3.Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, Tianjin, Peoples R China 4.Chinese Acad Sci, Univ Sci & Technol China, Hefei Inst Phys Sci, Hefei, Anhui, Peoples R China 5.Univ Sci & Technol China, Hefei, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiangyu,Yu, Chao,Yao, Jianming,et al. An Online Respiratory Quotient-Feedback Strategy of Feeding Yeast Extract for Efficient Arachidonic Acid Production by Mortierella alpina[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2018,5:11. |
| APA | Li, Xiangyu,Yu, Chao,Yao, Jianming,Wang, Zhiming,&Lu, Shuhuan.(2018).An Online Respiratory Quotient-Feedback Strategy of Feeding Yeast Extract for Efficient Arachidonic Acid Production by Mortierella alpina.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,5,11. |
| MLA | Li, Xiangyu,et al."An Online Respiratory Quotient-Feedback Strategy of Feeding Yeast Extract for Efficient Arachidonic Acid Production by Mortierella alpina".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 5(2018):11. |
入库方式： OAI收割
来源：合肥物质科学研究院
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