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  4. 中科院强磁场科学中心
Structural insights into the recognition of phosphorylated Hop1 by Mek1

文献类型：期刊论文

作者Xie, Changlin1,2; He, Chao3,4; Jiang, Yiyang2; Yu, Hailong2; Cheng, Lin2; Nshogoza, Gilbert2; Ala, Moududee Sayed2; Tian, Changlin1,2; Wu, Jihui2; Shi, Yunyu1,2
刊名ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
出版日期2018-10-01
卷号74页码:1027-1038
ISSN号2059-7983
关键词meiotic recombination Mek1 Hop1 FHA domain phosphointeraction
DOI10.1107/S2059798318011993
通讯作者Shi, Yunyu(yyshi@ustc.edu.cn) ; Li, Fudong(lifudong@ustc.edu.cn)
英文摘要The FHA domain-containing protein Mek1 is a meiosis-specific kinase that is involved in the regulation of interhomolog recombination in meiosis in Saccharomyces cerevisiae. The recruitment and activation of Mek1 require the phosphorylation of the chromosome axis protein Hop1 at Thr318 (pT318), which is necessary for recognition by the Mek1 FHA domain. Here, crystal structures of the Mek1 FHA domain in the apo state and in complex with the Hop1 pT318 peptide are presented, demonstrating that the hydrophobic residues Phe320 and Val321 at the pT+2 and pT+3 positions in the ligand contribute to the preferential recognition. It was further found that in Schizosaccharomyces pombe Mek1 FHA binds both pT15 in its N-terminal SQ/TQ cluster domain (SCD) and pT270 in the Hop1 SCD. The results revealed the structural basis for the preferential recognition of phosphorylated Hop1 by Mek1 in S. cerevisiae and facilitate the understanding of the interaction between the S. pombe Mek1 FHA domain and its binding targets.
WOS关键词FORKHEAD-ASSOCIATED DOMAIN ; MEIOTIC RECOMBINATION INITIATION ; STRAND BREAK REPAIR ; FHA DOMAIN ; SYNAPTONEMAL COMPLEX ; YEAST MEIOSIS ; SCHIZOSACCHAROMYCES-POMBE ; LINEAR ELEMENTS ; BUDDING YEAST ; INTERHOMOLOG RECOMBINATION
资助项目Chinese National Natural Science Foundation[31330018] ; Chinese National Natural Science Foundation[31500590] ; Chinese National Natural Science Foundation[31400643] ; Ministry of Science and Technology of China[2016YFA0500700] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Fundamental Research Funds for the Central Universities[WK2070000095]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Crystallography
语种英语
出版者INT UNION CRYSTALLOGRAPHY
WOS记录号WOS:000446420600010
资助机构Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/39513]  
专题合肥物质科学研究院_中科院强磁场科学中心
通讯作者Shi, Yunyu; Li, Fudong
作者单位1.Chinese Acad Sci, High Magnet Field Lab, 50 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, 96 Jinzhai Rd, Hefei 230026, Anhui, Peoples R China
3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei 230601, Anhui, Peoples R China
4.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Xie, Changlin,He, Chao,Jiang, Yiyang,et al. Structural insights into the recognition of phosphorylated Hop1 by Mek1[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,2018,74:1027-1038.
APA Xie, Changlin.,He, Chao.,Jiang, Yiyang.,Yu, Hailong.,Cheng, Lin.,...&Li, Fudong.(2018).Structural insights into the recognition of phosphorylated Hop1 by Mek1.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,74,1027-1038.
MLA Xie, Changlin,et al."Structural insights into the recognition of phosphorylated Hop1 by Mek1".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 74(2018):1027-1038.

入库方式： OAI收割

来源：合肥物质科学研究院

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