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Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy

文献类型：期刊论文

作者Xu, Difei1; Ma, Rongsheng1; Zhang, Jiahai1; Liu, Zhijun2; Wu, Bo4; Peng, Junhui1; Zhai, Yanan3; Gong, Qingguo1; Shi, Yunyu1; Wu, Jihui1
刊名JOURNAL OF PHYSICAL CHEMISTRY LETTERS
出版日期2018-07-19
卷号9期号:14页码:4020-4028
ISSN号1948-7185
DOI10.1021/acs.jpclett.8b01440
通讯作者Zhang, Zhiyong(zzyzhang@ustc.edu.cn) ; Ruan, Ke(kruan@ustc.edu.cn)
英文摘要The 11 zinc fingers (ZFs) of the transcription factor CTCF play a versatile role in the regulation of gene expression. CTCF binds to numerous genomic sites to form chromatin loops and topologically associated domains and thus mediates the 3D architecture of chromatin. Although CTCF inter-ZF plasticity is essential for the recognition of multiple genomic sites, the dynamic nature of its 11 ZFs remains unknown. We assigned the chemical shifts of the CTCF ZFs 1-11 and solved the solution structures of each ZF. NMR backbone dynamics, residual dipolar couplings, and small-angle X-ray scattering experiments suggest a high inter-ZF plasticity of the free-form ZFs 1-11. As exemplified by two different protocadherin DNA sequences, the titration of DNAs to N-15-labeled CTCF ZFs 1-11 enabled systematic mapping of binding of CTCF ZFs to various chromatin sites. Our work paves the way for illustrating the molecular basis of the versatile DNA recognized by CTCF and has interesting implications for its conformational transition during DNA binding.
WOS关键词RESIDUAL DIPOLAR COUPLINGS ; BIOLOGICAL MACROMOLECULES ; FUNCTIONAL-ORGANIZATION ; CHROMATIN INTERACTIONS ; MAMMALIAN GENOMES ; BINDING ; COHESIN ; GENE ; ALIGNMENT ; PROTEINS
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08030302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDPB10] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020355] ; Ministry of Science and Technology of China[2016YFA0500700] ; Ministry of Science and Technology of China[2014CB910604] ; National Natural Science Foundation of China[U1632153] ; National Natural Science Foundation of China[21573205] ; National Natural Science Foundation of China[31630039] ; Fundamental Research Funds for the Central Universities[WK2070080002] ; Fundamental Research Funds for the Central Universities[WK2060190086]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种英语
WOS记录号WOS:000448083300033
出版者AMER CHEMICAL SOC
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/39922]  
专题合肥物质科学研究院_中科院强磁场科学中心
通讯作者Zhang, Zhiyong; Ruan, Ke
作者单位1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
2.Chinese Acad Sci, Shanghai Adv Res Inst, ZhangJiang Lab, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China
3.Shanghai Jiao Tong Univ, Ctr Comparat Biomed,Sch Life Sci & Biotechnol, MOE Key Lab Syst Biomed,SCSB,State Key Lab On Cog, Inst Syst Biomed,Collaborat Innovat Ctr Syst Biom, Shanghai 200240, Peoples R China
4.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Difei,Ma, Rongsheng,Zhang, Jiahai,et al. Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2018,9(14):4020-4028.
APA Xu, Difei.,Ma, Rongsheng.,Zhang, Jiahai.,Liu, Zhijun.,Wu, Bo.,...&Ruan, Ke.(2018).Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,9(14),4020-4028.
MLA Xu, Difei,et al."Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 9.14(2018):4020-4028.

入库方式： OAI收割

来源：合肥物质科学研究院

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