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  4. 技术生物与农业工程研究所
Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells

文献类型：期刊论文

作者Yang, Xiaoyao1,2; Xu, Shengmin1,4; Su, Yao1,2; Chen, Biao1,2; Yuan, Hang1,4; Xu, An1,4; Wu, Lijun1,3,4
刊名RADIATION RESEARCH
出版日期2018-11-01
卷号190期号:5页码:494-503
ISSN号0033-7587
DOI10.1667/RR15073.1
通讯作者Xu, Shengmin(shmxu@mail.ustc.edu.cn) ; Wu, Lijun(ljw@ipp.ac.cn)
英文摘要Connexin molecules are an important component of the gap junction, with connexin43 (Cx43) being the most abundantly expressed type. Src is a nonreceptor tyrosine-protein kinase that affects Cx43 activity by multiple mechanisms. However, it is not clear how Src regulates Cx43 to influence radiation-induced bystander effects (RIBEs). In this study, we demonstrated that Cx43 on Tyr265 was phosphorylated by activated Src in a-irradiated HepG2 cells, with the total expression of Cx43 unchanged. After inhibition of Cx43 phosphorylation in irradiated cells, the frequency of gamma-H2AX foci formation in adjacent nonirradiated bystander cells was significantly enhanced. Furthermore, this study showed that autophagy regulated the activity of Src and phosphorylation of Cx43, and the level of autophagy was correlated with the radiation-induced reactive oxygen species (ROS). These results suggest that ROS and autophagy play an important role in regulating the Src-Cx43 axis to affect the RIBEs. Our findings provide new insights into the Cx43-mediated gap junction intercellular communication, as well as the underlying mechanism of RIBEs. (C) 2018 by Radiation Research Societe
WOS关键词PROTEIN-KINASE-C ; IONIZING-RADIATION ; TYROSINE PHOSPHORYLATION ; MOLECULAR-BASIS ; V-SRC ; CONNEXIN-43 ; EXPRESSION ; PERMEABILITY ; MECHANISM ; PATHWAY
资助项目International Partnership Program of Chinese Academy of Sciences[116134KYSB20160084] ; National Natural Science Foundation of China[31470829] ; National Natural Science Foundation of China[81273004] ; National Natural Science Foundation of China[31670860] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2016FXCX005] ; ITER program[2014GB112006]
WOS研究方向Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:000452084900005
出版者RADIATION RESEARCH SOC
资助机构International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/40442]  
专题合肥物质科学研究院_技术生物与农业工程研究所
通讯作者Xu, Shengmin; Wu, Lijun
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, POB 1138, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
3.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China
4.Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Xiaoyao,Xu, Shengmin,Su, Yao,et al. Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells[J]. RADIATION RESEARCH,2018,190(5):494-503.
APA Yang, Xiaoyao.,Xu, Shengmin.,Su, Yao.,Chen, Biao.,Yuan, Hang.,...&Wu, Lijun.(2018).Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells.RADIATION RESEARCH,190(5),494-503.
MLA Yang, Xiaoyao,et al."Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells".RADIATION RESEARCH 190.5(2018):494-503.

入库方式： OAI收割

来源：合肥物质科学研究院

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