The 1.6 angstrom resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer
文献类型：期刊论文
| 作者 | Tao WB(陶文斌) ; Gao ZQ(高增强) ; Gao ZY(高增亚) ; Zhou JH(周佳海) ; Huang ZX(黄仲贤) ; Dong YH(董宇辉) ; Yu SN(余绍宁) |
| 刊名 | Int. J. Biol. Macromol.
 |
| 出版日期 | 2011 |
| 卷号 | 48期号:3页码:459-465 |
| ISSN号 | 0141-8130 |
| 其他题名 | 降解物基因活化蛋白D38L突变体与两种cAMP的1.6埃分辨率结构报道 |
| 通讯作者 | 董宇辉 ; 余绍宁 |
| 英文摘要 | The X-ray crystal structure of the cAMP-liganded D138L mutant of Escherichia coil catabolite gene activator protein (CAP) was determined at a resolution of 1.66 angstrom. This high resolution crystal structure reveals four cAMP binding sites in the homodimer. Two anti conformations of cAMPs (anti-CAMP) locate between the beta-barrel and the C-helix of each subunit; two syn conformations of cAMPs (syn-cAMP) bind on the surface of the C-terminal domain. With two syn-cAMP molecules bound, the D138L CAP is highly symmetrical with both subunits assuming a "closed" conformation. These differences make the hinge region of the mutant more flexible. Protease susceptibility measurements indicate that D138L is more susceptible to proteases than that of wild type (WT) CAP. The results of protein dynamic experiments (H/D exchange measurements) indicate that the structure of D138L mutant is more dynamic than that of WT CAP, which may impact the recognition of specific DNA sequences. (C) 2011 Elsevier B.V. All rights reserved. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.ijbiomac.2011.01.009 |
| 语种 | 英语 |
| WOS记录号 | WOS:000289021400013 |
| 公开日期 | 2013-08-21 |
| 源URL | [http://202.127.28.38/handle/331003/27831]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Tao WB,Gao ZQ,Gao ZY,et al. The 1.6 angstrom resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer[J]. Int. J. Biol. Macromol.,2011,48(3):459-465. |
| APA | 陶文斌.,高增强.,高增亚.,周佳海.,黄仲贤.,...&余绍宁.(2011).The 1.6 angstrom resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer.Int. J. Biol. Macromol.,48(3),459-465. |
| MLA | 陶文斌,et al."The 1.6 angstrom resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer".Int. J. Biol. Macromol. 48.3(2011):459-465. |
入库方式： OAI收割
来源：上海有机化学研究所
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