Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers
文献类型：期刊论文
| 作者 | Liu C(刘超) ; Kang JW(康经武) |
| 刊名 | J. Chromatogr. A
 |
| 出版日期 | 2012 |
| 卷号 | 1238页码:146-151 |
| ISSN号 | 0021-9673 |
| 其他题名 | 聚电解质动态涂层用于亲和毛细管电泳前沿分析方法 |
| 通讯作者 | 康经武 |
| 英文摘要 | An improved CE-FA method using a polyelectrolyte multilayer (PEML) coated capillary is described. The coating was simply created by alternatively flushing the capillary with positively charged polyelectrolyte polybrene (HDB) and negatively charged polyelectrolyte dextran sulfate (DS). Human serum albumin (HSA) and 6 drugs were selected as an experimental model to evaluate such an improvement. The PEML coating was demonstrated to effectively reduce the protein adsorption on the capillary wall. Compared with the uncoated capillary, the repeatability (RSD%) of the EOF mobility and the plateau height of free drug were improved significantly from 3.7% to 0.51% and from 0.61% to 0.34%, respectively. No interference of the coating on the measurement of protein-drug binding parameters was observed. (C) 2012 Elsevier B.V. All rights reserved. |
| 学科主题 | 分析化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.chroma.2012.03.043 |
| 语种 | 英语 |
| WOS记录号 | WOS:000303843000018 |
| 公开日期 | 2013-08-29 |
| 源URL | [http://202.127.28.38/handle/331003/28669]  |
| 专题 | 上海有机化学研究所_分析化学研究室 |
| 推荐引用方式 GB/T 7714 | Liu C,Kang JW. Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers[J]. J. Chromatogr. A,2012,1238:146-151. |
| APA | 刘超,&康经武.(2012).Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers.J. Chromatogr. A,1238,146-151. |
| MLA | 刘超,et al."Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers".J. Chromatogr. A 1238(2012):146-151. |
入库方式： OAI收割
来源：上海有机化学研究所
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