Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway
文献类型：期刊论文
| 作者 | Yang ZZ(杨中正) ; Cody Vild ; JU JIAYING ; ZHANG XU ; LIU JIANPING ; SHEN JIE ; ZHAO BIN ; Lan WX(蓝文贤) ; GONG FUCHUN ; LIU MAILI ; Cao CY(曹春阳) ; XU ZHAOHUI |
| 刊名 | J. Biol. Chem.
 |
| 出版日期 | 2012 |
| 卷号 | 287期号:52页码:43899-43908 |
| ISSN号 | 0021-9258 |
| 其他题名 | ESCRT-III体系复合物Vta1NTD-Vps60溶液结构测定 |
| 通讯作者 | 曹春阳 ; XU ZHAOHUI |
| 英文摘要 | The AAA-ATPase Vps4 is critical for function of the multivesicular body sorting pathway, which impacts cellular phenomena ranging from receptor down-regulation to viral budding to cytokinesis. Vps4 activity is stimulated by the interaction between Vta1 and Vps60, but the structural basis for this interaction is unclear. The fragment Vps60(128-186) was reported to display the full activity of Vps60. Vta1 interacts with Vps60 using its N-terminal domain (Vta1NTD). In this work, the structure of Vps60(128-186) in complex with Vta1NTD was determined using NMR techniques, demonstrating a novel recognition mode of the microtubule-interacting and transport (MIT) domain in which Vps60(128-186) interacts with Vta1NTD through helices alpha 4' and alpha 5', extending over Vta1NTD MIT2 domain helices 1-3. The Vps60 binding does not result in Vta1 conformational changes, further revealing the fact that Vps4 ATPase is enhanced by the interaction between Vta1 and Vps60 in an unanticipated manner. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1074/jbc.M112.390724 |
| 语种 | 英语 |
| WOS记录号 | WOS:000312940800066 |
| 公开日期 | 2013-08-23 |
| 源URL | [http://202.127.28.38/handle/331003/28489]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang ZZ,Cody Vild,JU JIAYING,et al. Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway[J]. J. Biol. Chem.,2012,287(52):43899-43908. |
| APA | 杨中正.,Cody Vild.,JU JIAYING.,ZHANG XU.,LIU JIANPING.,...&XU ZHAOHUI.(2012).Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway.J. Biol. Chem.,287(52),43899-43908. |
| MLA | 杨中正,et al."Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway".J. Biol. Chem. 287.52(2012):43899-43908. |
入库方式： OAI收割
来源：上海有机化学研究所
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