In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O
文献类型：期刊论文
| 作者 | Peng C(彭超) ; Tang YM(唐玉敏) ; Li L(李磊) ; Ding W(丁伟) ; Deng W(邓玮) ; Pu JY(蒲津越) ; Liu W(刘文) ; Tang GL(唐功利) |
| 刊名 | Sci. China-Chem.
 |
| 出版日期 | 2012 |
| 卷号 | 55期号:1页码:90-97 |
| ISSN号 | 1674-7291 |
| 其他题名 | 通过鉴定番红霉素O的结构体内研究SfmO2在番红霉素A生物合成中的功能 |
| 通讯作者 | 唐功利 |
| 英文摘要 | Saframycin A (SFM-A), a tetrahydroisoquinoline antibiotic isolated from Streptomyces lavendulae, shows potent anti-proliferation activities against a variety of tumor cell lines, and shares the core structure with ecteinascidin 743 (ET-743), the anticancer drug for soft-tissue sarcoma. Characterization of the SFM-A biosynthetic gene cluster revealed three nonribosomal peptide synthetase genes and a series of genes encoding oxygenases. To investigate the function of sfmO2 gene, encoding a FAD-dependent monooxygenase/hydroxylase, we constructed the gene replacement mutant (Delta sfmO2) strain S. lavendulae TL2007 and the corresponding gene complementation mutant strain S. lavendulae TL2008. A novel compound, SFM-O, was isolated from the Delta sfmO2 replacement mutant strain and its structure was characterized by comparison to the HRMS and NMR spectra of SFM-A. These findings indicated that SfmO2 is responsible for the oxidation of ring A in the biosynthetic pathway of SFM-A, and the new compound SFM-O could be considered as an advanced intermediate in the semisynthesis of ET-743. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1007/s11426-011-4450-4 |
| 语种 | 英语 |
| WOS记录号 | WOS:000298968200012 |
| 公开日期 | 2013-08-23 |
| 源URL | [http://202.127.28.38/handle/331003/28551]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Peng C,Tang YM,Li L,et al. In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O[J]. Sci. China-Chem.,2012,55(1):90-97. |
| APA | 彭超.,唐玉敏.,李磊.,丁伟.,邓玮.,...&唐功利.(2012).In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O.Sci. China-Chem.,55(1),90-97. |
| MLA | 彭超,et al."In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O".Sci. China-Chem. 55.1(2012):90-97. |
入库方式： OAI收割
来源：上海有机化学研究所
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