Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages
文献类型：期刊论文
| 作者 | Guo, XM; Wang, H; Li, Y; Leng, XP; Huang, WW; Ma, YB; Xu, T; Qi, XP |
| 刊名 | IMMUNOLOGY AND CELL BIOLOGY
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| 出版日期 | 2019 |
| 卷号 | 97期号:1页码:92-96 |
| ISSN号 | 0818-9641 |
| 英文摘要 | The commercial transfection reagent Lipofectamine has been widely used for cytoplasmic delivery of nucleic acids and for cytosolic engagement with intracellular innate immune sensors to trigger type I interferon (IFN) production. However, the effect of Lipofectamine alone on type I IFN response has not been studied in detail. Here, we show that Lipofectamine induced type I IFN signaling in both RAW 264.7 macrophage-like cells and primary bone marrow-derived macrophages. Type I IFN induction was dependent on interferon regulatory factor (IRF)3 and IRF7 and partially required the toll/interleukin-1 receptor-domain-containing adapter-inducing interferon-beta. In contrast, the transfection reagent Xfect did not activate type I IFN signaling. Our study highlights the potential confounding experimental interpretation when using Lipofectamine-based transfection for delivering intracellular ligands and provides important insights into lipid signaling in innate immune responses. |
| 语种 | 英语 |
| 源URL | [http://ir.kiz.ac.cn:8080/handle/152453/12535]  |
| 专题 | 昆明动物研究所_病原菌感染与宿主免疫机制 |
| 推荐引用方式 GB/T 7714 | Guo, XM,Wang, H,Li, Y,et al. Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages[J]. IMMUNOLOGY AND CELL BIOLOGY,2019,97(1):92-96. |
| APA | Guo, XM.,Wang, H.,Li, Y.,Leng, XP.,Huang, WW.,...&Qi, XP.(2019).Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages.IMMUNOLOGY AND CELL BIOLOGY,97(1),92-96. |
| MLA | Guo, XM,et al."Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages".IMMUNOLOGY AND CELL BIOLOGY 97.1(2019):92-96. |
入库方式： OAI收割
来源：昆明动物研究所
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