Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing
文献类型：期刊论文
| 作者 | Yang, LX; Yang, YD; Yuan, JM; Sun, Y; Dai, JP; Su, B |
| 刊名 | CEREBRAL CORTEX
 |
| 出版日期 | 2019 |
| 卷号 | 29期号:2页码:838-851 |
| ISSN号 | 1047-3211 |
| 英文摘要 | The von Economo neurons (VENs) are specialized large bipolar projection neurons with restricted distribution in the human brain, and they are far more abundant in humans than in non-human primates. However, VEN functions remain elusive due to the difficulty of isolating VENs and dissecting their connections in the brain. Here, we combined laser-capture-microdissection with RNA sequencing to describe the transcriptomic profile of VENs from human anterior cingulate cortex (ACC). Using pyramidal neurons as reference cells, we identified 344 genes with VEN-associated expression differences, including 215 higher and 129 lower expression genes. Functional enrichment and protein-protein interaction network analyses showed that these genes with VEN-associated expression differences are involved in VEN morphogenesis and functions, such as dendrite branching and axon myelination, and many of them are associated with human social-emotional disorders. With the use of in situ hybridization and immunohistochemistry assays, we validated four novel VEN markers (VAT1L, CHST8, LYPD1, and SULF2). Collectively, we generated a full-spectrum expression profile of VENs from human ACC, greatly enlarging the pool of genes with VEN-associated expression differences that can help researchers to understand the role of VENs in normal and disordered human brains. |
| 语种 | 英语 |
| 源URL | [http://ir.kiz.ac.cn:8080/handle/152453/12548]  |
| 专题 | 昆明动物研究所_比较基因组学 |
| 推荐引用方式 GB/T 7714 | Yang, LX,Yang, YD,Yuan, JM,et al. Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing[J]. CEREBRAL CORTEX,2019,29(2):838-851. |
| APA | Yang, LX,Yang, YD,Yuan, JM,Sun, Y,Dai, JP,&Su, B.(2019).Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing.CEREBRAL CORTEX,29(2),838-851. |
| MLA | Yang, LX,et al."Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing".CEREBRAL CORTEX 29.2(2019):838-851. |
入库方式： OAI收割
来源：昆明动物研究所
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