Macrofungus is defined as the fungus that grows an observable sporocarp. The sporocarps of manyspecies are commonly called mushrooms and consumed by people all around the world as food and/or medicine. Most macrofungi belong to the divisions Basidiomycetes and Ascomycetes, which areestimated to contain more than 80,000 species in total. We report the draft genome assemblies ofmacrofungi (83 Basidiomycetes species and 7 Ascomycetes species) based on Illumina sequencing. Thegenome sizes of these species ranged from 27.4 Mb (Hygrophorus russula) to 202.2 MB (Chroogomphusrutilus). The numbers of protein-coding genes were predicted in the range of 9,511 (Hygrophorusrussula) to 52,289 (Craterellus lutescens). This study provides the largest genomic dataset formacrofungi species. This resource will facilitate the artificial cultivation of edible mushrooms and thediscovery of novel drug candidates.