BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori
文献类型：期刊论文
| 作者 | Kangkang Niu; Xiaojuan Zhang; Hui Xiang; Sichun Zheng; Lin Liu; Lihua Huang; Baojuan Zeng; Sheng Li; Huimin Deng; Qingyou Xia |
| 刊名 | Nucleic Acids Research
 |
| 出版日期 | 2018 |
| 卷号 | 46期号:4页码:1710–1723 |
| DOI | 10.1093/nar/gkx1207 |
| 英文摘要 | Guanine-rich and cytosine-rich DNA can form four-stranded DNA secondary structures called G-quadruplex (G4) and i-motif, respectively. Thesestructures widely exist in genomes and play impor-tant roles in transcription, replication, translationand protection of telomeres. In this study, G4 andi-motif structures were identified in the promoter ofthe transcription factor gene BmPOUM2 , which reg-ulates the expression of the wing disc cuticle pro-tein gene ( BmWCP4 ) during metamorphosis. Disrup-tion of the i-motif structure by base mutation, anti-sense oligonucleotides (ASOs) or inhibitory ligandsresulted in significant decrease in the activity of theBmPOUM2 promoter. A novel i-motif binding pro-tein (BmILF) was identified by pull-down experiment.BmILF specifically bound to the i-motif and activatedthe transcription of BmPOUM2 . The promoter activityof BmPOUM2 was enhanced when BmILF was over-expressed and decreased when BmILF was knocked-down by RNA interference. This study for the firsttime demonstrated that BmILF and the i-motif struc-ture participated in the regulation of gene transcrip-tion in insect metamorphosis and provides new in-sights into the molecular mechanism of the sec-ondary structures in epigenetic regulation of genetranscription. |
| 语种 | 英语 |
| 资助机构 | Chinese National Natural Science Foundation [31330071,31672494,31720103916,2012CB114602].Fundingforopenaccess charge: South China Normal University.Conflict of interest statement. None declared. ; Chinese National Natural Science Foundation [31330071,31672494,31720103916,2012CB114602].Fundingforopenaccess charge: South China Normal University.Conflict of interest statement. None declared. ; Chinese National Natural Science Foundation [31330071,31672494,31720103916,2012CB114602].Fundingforopenaccess charge: South China Normal University.Conflict of interest statement. None declared. ; Chinese National Natural Science Foundation [31330071,31672494,31720103916,2012CB114602].Fundingforopenaccess charge: South China Normal University.Conflict of interest statement. None declared. |
| 源URL | [http://159.226.149.26:8080/handle/152453/12134]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 通讯作者 | qlfeng@scnu.edu.cn |
| 作者单位 | 1.Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China 4.Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA 5.Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA |
| 推荐引用方式 GB/T 7714 | Kangkang Niu,Xiaojuan Zhang,Hui Xiang,et al. BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori[J]. Nucleic Acids Research,2018,46(4):1710–1723. |
| APA | Kangkang Niu.,Xiaojuan Zhang.,Hui Xiang.,Sichun Zheng.,Lin Liu.,...&qlfeng@scnu.edu.cn.(2018).BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.Nucleic Acids Research,46(4),1710–1723. |
| MLA | Kangkang Niu,et al."BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori".Nucleic Acids Research 46.4(2018):1710–1723. |
入库方式： OAI收割
来源：昆明动物研究所
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