Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis
文献类型：期刊论文
| 作者 | He SM1; Song WL1; Cai J4; Zhang JJ1; Zhang GH1; Yang JL6; Yang SC*1; Fan W*1; Cong K1; Wang X2,5 |
| 刊名 | Scientific Reports
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:x页码:e9119 |
| 其他题名 | shengchaoyang@163.com;fanwei1128@aliyun.com |
| 英文摘要 | Dactylicapnos scandens (D. Don) Hutch (Papaveraceae) is a well-known traditional Chinese herb used for treatment of hypertension, inflammation, bleeding and pain for centuries. Although the major bioactive components in this herb are considered as isoquinoline alkaloids (IQAs), little is known about molecular basis of their biosynthesis. Here, we carried out transcriptomic analysis of roots, leaves and stems of D. scandens, and obtained a total of 96,741 unigenes. Based on gene expression and phylogenetic relationship, we proposed the biosynthetic pathways of isocorydine, corydine, glaucine and sinomenine, and identified 67 unigenes encoding enzymes potentially involved in biosynthesis of IQAs in D. scandens. High performance liquid chromatography analysis demonstrated that while isocorydine is the most abundant IQA in D. scandens, the last O-methylation biosynthesis step remains unclear. Further enzyme activity assay, for the first time, characterized a gene encoding O- methyltransferase (DsOMT), which catalyzes O-methylation at C7 of (S)-corytuberine to form isocorydine. We also identified candidate transcription factor genes belonging to WRKY and bHLH families that may be involved in the regulation of IQAs biosynthesis. Taken together, we first provided valuable genetic information for D. scandens, shedding light on candidate genes involved in IQA biosynthesis, which will be critical for further gene functional characterization. |
| 语种 | 英语 |
| 资助机构 | his work was supported by the pilot project for establishing new socialized service system by agricultural science and education combination in Yunnan Province (Medical Plant Unit) (2014NG003); the project of young and middle-aged talent of Yunnan Province (2014HB011). ; his work was supported by the pilot project for establishing new socialized service system by agricultural science and education combination in Yunnan Province (Medical Plant Unit) (2014NG003); the project of young and middle-aged talent of Yunnan Province (2014HB011). |
| 源URL | [http://159.226.149.26:8080/handle/152453/12034]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan, National& Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, 650201, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China 3.Province Key Laboratory, Biological Big Data College, Yunan Agricultural University, Kunming, 650201, China 4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, 999078, China 5.Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China 6.State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China. |
| 推荐引用方式 GB/T 7714 | He SM,Song WL,Cai J,et al. Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis[J]. Scientific Reports,2017,7(x):e9119. |
| APA | He SM.,Song WL.,Cai J.,Zhang JJ.,Zhang GH.,...&Dong Y.(2017).Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis.Scientific Reports,7(x),e9119. |
| MLA | He SM,et al."Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis".Scientific Reports 7.x(2017):e9119. |
入库方式： OAI收割
来源：昆明动物研究所
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