Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner
文献类型：期刊论文
| 作者 | Zheng C1,2; Mao HL3; Cao Y*1; caoy@mail.kiz.ac.cn |
| 刊名 | Scientific Reports
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:X页码:e3769 |
| 英文摘要 | Symplekin is a multifunctional protein that localizes to both tight junctions and the nucleus in polarized epithelial cells, with confirmed roles in mRNA maturation, transcriptional modulation and tight-junction assembly. However, the mechanisms governing its subcellular distribution and related functions remain unclear. In this study, we found that symplekin primarily localizes to the nuclei of cultured dedifferentiated colorectal cancer cells, and nuclear symplekin showed higher phosphorylation and binding affinity with YBX3 than its membrane fraction. Moreover, the accumulation of nuclear symplekin promoted cell proliferation and dedifferentiation as well as β-catenin transactivation in vitro. Nuclear symplekin acts as a transcriptional co-activator for the expression of many cell cycle-related genes. Furthermore, extracellular signal-regulated kinase (ERK) phosphorylated symplekin at T1257 to facilitate its nuclear accumulation upon epidermal growth factor (EGF) stimulation. Meanwhile, reduction of total symplekin also induced certain epithelial-mesenchymal transition features in HT-29 cells. Taken together, our results confirm the coordinated roles of symplekin in cell junctions and gene transcription, which are related to its subcellular localization. The significance of nuclear symplekin in tumorigenesis is also highlighted, and ERK-dependent phosphorylation represents a mechanism for its subcellular sorting. |
| 语种 | 英语 |
| 资助机构 | The study was supported by the National Natural Science Foundation of China (No. 30800407 and 81201667), the 100 Talents Program of the Chinese Academy of Sciences (Grant Cao), and the Science and Technology Department, Yunnan Province, China (Y103951111). ; The study was supported by the National Natural Science Foundation of China (No. 30800407 and 81201667), the 100 Talents Program of the Chinese Academy of Sciences (Grant Cao), and the Science and Technology Department, Yunnan Province, China (Y103951111). ; The study was supported by the National Natural Science Foundation of China (No. 30800407 and 81201667), the 100 Talents Program of the Chinese Academy of Sciences (Grant Cao), and the Science and Technology Department, Yunnan Province, China (Y103951111). ; The study was supported by the National Natural Science Foundation of China (No. 30800407 and 81201667), the 100 Talents Program of the Chinese Academy of Sciences (Grant Cao), and the Science and Technology Department, Yunnan Province, China (Y103951111). |
| 源URL | [http://159.226.149.26:8080/handle/152453/11758]  |
| 专题 | 昆明动物研究所_分子病理学 |
| 通讯作者 | caoy@mail.kiz.ac.cn |
| 作者单位 | 1.Laboratory of Molecular and Experimental Pathology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China. 3.Department of Anesthesiology and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China |
| 推荐引用方式 GB/T 7714 | Zheng C,Mao HL,Cao Y*,et al. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner[J]. Scientific Reports,2017,7(X):e3769. |
| APA | Zheng C,Mao HL,Cao Y*,&caoy@mail.kiz.ac.cn.(2017).Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner.Scientific Reports,7(X),e3769. |
| MLA | Zheng C,et al."Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner".Scientific Reports 7.X(2017):e3769. |
入库方式： OAI收割
来源：昆明动物研究所
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