Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase
文献类型：期刊论文
| 作者 | Wei JF1,2; Mo YZ1; Qiao LY1; Wei XL1; Chen HQ1; Xie H1; Fu YL1; Wang WY2; Xiong YL2; He SH[*]1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 38期号:4页码:510-520 |
| 关键词 | Histamine Metalloproteinase Mast Cell Naja Atra G-protein Coupled Receptors |
| ISSN号 | 1357-2725 |
| 英文摘要 | Poisonous snakebite wound is a popular disease worldwide. However, the pathogenesis remains unclear. In the present study, a novel metalloproteinase atrahagin in Chinese cobra (Naja atra) snake venom was purified, using heparin-sepharose followed by Superdex 75 gel filtration chromatography. Apart from its alpha-fibrinogenase activity, atrahagin potently activated human colon, lung and tonsil mast cells with the net histamine release being 25.9 +/- 4.4, 17.0 +/- 1.9, 13.2 +/- 3.6%, respectively. Time course studies revealed that the peak histamine release induced by atrahagin occurred at 12, 12 and 8 min following incubation of the enzyme with colon, lung and tonsil mast cells, respectively. The response of mast cells to atrahagin was abolished by preincubation of the cells with metabolic inhibitors or pertussis toxin, and by removal of Ca2+ and Mg2+ from the challenge buffer. In conclusion, activation of human mast cells by atrahagin indicated that the enzyme might contribute to the pathogenesis of snakebite wound. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 资助机构 | This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2817]  |
| 专题 | 昆明动物研究所_其他 |
| 通讯作者 | weijifu@hotmail.com; shoahenghe@hotmail.com |
| 作者单位 | 1.Allergy and Inflammation Research Institute, The Shantou University Medical College, Shantou, Guangdong Province 515041, China 2.Department of Animal Toxicology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China |
| 推荐引用方式 GB/T 7714 | Wei JF,Mo YZ,Qiao LY,et al. Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2006,38(4):510-520. |
| APA | Wei JF.,Mo YZ.,Qiao LY.,Wei XL.,Chen HQ.,...&shoahenghe@hotmail.com.(2006).Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,38(4),510-520. |
| MLA | Wei JF,et al."Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 38.4(2006):510-520. |
入库方式： OAI收割
来源：昆明动物研究所
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