N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)
文献类型：期刊论文
| 作者 | Wei JF1,2; Wei XL1; Chen QY1; Huang T1; Qiao LY1; Wang WY2; Xiong YL2; He SH*1; shoahenghe@hotmail.com |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2006 |
| 卷号 | 1760期号:3页码:462-471 |
| 关键词 | Molecular Cloning Phylogenetic Analysis Myotoxicity Phospholipase a(2) Snake Venom Protobothrops Mucrosquamatus |
| ISSN号 | 0304-4165 |
| 英文摘要 | A novel phospholipase A(2) (PLA(2)) with Asn at its site 49 was purified from the snake venom of Protobothrops mucrosquamatus by using SP-Sephadex C25, Superdex 75, Heparin-Sepharose (FF) and HPLC reverse-phage C-18 chromatography and designated as TM-N49. It showed a molecular mass of 13.875 kDa on MALDI-TOF. TM-N49 does not possess enzymatic, hemolytic and hemorrhagic activities. It fails to induce platelet aggregation by itself, and does not inhibit the platelet aggregation induced by ADP. However, it exhibits potent myotoxic activity causing inflammatory cell infiltration, severe myoedema, myonecrosis and myolysis in the gastrocnemius muscles of BALB/c mice. Phylogenetic analysis found that that TM-N49 combined with two phospholipase A(2)s from Trimeresurus stejnegeri, TsR6 and CTs-R6 cluster into one group. Structural and functional analysis indicated that these phospholipase A(2)s are distinct from the other subgroups (D49 PLA(2), S49 PLA(2) and K49 PLA(2)) and represent a unique subgroup of snake venom group II PLA(2), named N49 PLA(2) subgroup. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 资助机构 | This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2815]  |
| 专题 | 昆明动物研究所_其他 |
| 通讯作者 | shoahenghe@hotmail.com |
| 作者单位 | 1.Allergy and Inflammation Research Institute, the Shantou University Medical College, Shantou, Guangdong, 515031, China 2.Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China |
| 推荐引用方式 GB/T 7714 | Wei JF,Wei XL,Chen QY,et al. N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2006,1760(3):462-471. |
| APA | Wei JF.,Wei XL.,Chen QY.,Huang T.,Qiao LY.,...&shoahenghe@hotmail.com.(2006).N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2).BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1760(3),462-471. |
| MLA | Wei JF,et al."N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1760.3(2006):462-471. |
入库方式： OAI收割
来源：昆明动物研究所
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