T cells have been successfully applied to cancer immunotherapy. However, a challenge is an expansion of T cells from cyno-molgus monkey, which is a valuable non-human primate model for Tcell therapy transferring to the clinic. Here, we comparedseveral strategies to expand cynomolgus monkey T cell and developed an appropriate method. Our study demonstrated that100 ng/ml CD3 mAb+1% PHA+ 1000 U/ml IL2 therapy significantly expanded peripheral blood CD3+ T cells withoutcompromising T cell phenotype in vitro. The results of this study could be used for T cell remodeling, which has significanttherapeutic potential in Chimeric Antigen Receptor-T (CAR-T) cell immunotherapy.