Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix)
文献类型:期刊论文
作者 | Liu, Haiyang1,2; Pang, Meixia1,2; Yu, Xiaomu2; Zhou, Ying1,2; Tong, Jingou2; Fu, Beide2 |
刊名 | DNA RESEARCH |
出版日期 | 2018-06-01 |
卷号 | 25期号:3页码:257-264 |
ISSN号 | 1340-2838 |
关键词 | sex-specific markers XX/XY sex determination system bighead carp and silver carp |
DOI | 10.1093/dnares/dsx054 |
英文摘要 | Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in bighead carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in bighead carp and silver carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in bighead carp and silver carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large carp species. |
WOS关键词 | YELLOW CATFISH ; CHROMOSOME EVOLUTION ; PROVIDES INSIGHTS ; ARISTICHTHYS-NOBILIS ; ONCORHYNCHUS-MYKISS ; GENOME SEQUENCE ; CYPRINUS-CARPIO ; LINKED MARKERS ; RAINBOW-TROUT ; COMMON CARP |
资助项目 | NSFC[31472268] ; NSFC[31502153] ; FEBL[2016FBZ05] ; DAC[2009045] |
WOS研究方向 | Genetics & Heredity |
语种 | 英语 |
出版者 | OXFORD UNIV PRESS |
WOS记录号 | WOS:000439825000003 |
资助机构 | NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC |
源URL | [http://ir.ihb.ac.cn/handle/342005/34509] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Fu, Beide |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Haiyang,Pang, Meixia,Yu, Xiaomu,et al. Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix)[J]. DNA RESEARCH,2018,25(3):257-264. |
APA | Liu, Haiyang,Pang, Meixia,Yu, Xiaomu,Zhou, Ying,Tong, Jingou,&Fu, Beide.(2018).Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix).DNA RESEARCH,25(3),257-264. |
MLA | Liu, Haiyang,et al."Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix)".DNA RESEARCH 25.3(2018):257-264. |
入库方式: OAI收割
来源:水生生物研究所
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