A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens
文献类型：期刊论文
| 作者 | Pu, Xiaojun4,5 ; Liu, Lina3,4,5; Li, Ping3,4,5; Huo, Heqiang2; Dong, Xiumei4,5; Xie, Kabin1; Yang, Hong3,4,5; Liu, Li4,5
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| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2019-09-06 |
| 页码 | 10 |
| 关键词 | CRISPR Cas12a CRISPR Cas9 genome editing Physcomitrella patens technical advance |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.14478 |
| 通讯作者 | Liu, Li(liulia@mail.kib.ac.cn) |
| 英文摘要 | Due to their high efficiency, specificity, and flexibility, programmable nucleases, such as those of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a (Cpf1) system, have greatly expanded the applicability of editing the genomes of various organisms. Genes from different gene families or genes with redundant functions in the same gene family can be examined by assembling multiple CRISPR RNAs (crRNAs) in a single vector. However, the activity and efficiency of CRISPR/Cas12a in the non-vascular plant Physcomitrella patens are largely unknown. Here, we demonstrate that LbCas12a together with its mature crRNA can target multiple loci simultaneously in P. patens with high efficiency via co-delivery of LbCas12a and a crRNA expression cassette in vivo. The mutation frequencies induced by CRISPR/LbCas12a at a single locus ranged from 26.5 to 100%, with diverse deletions being the most common type of mutation. Our method expands the repertoire of genome editing tools available for P. patens and facilitates the creation of loss-of-function mutants of multiple genes from different gene families. |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000485845800001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/68704]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 通讯作者 | Liu, Li |
| 作者单位 | 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China 2.Univ Florida, Dept Environm Hort, Mid Florida Res & Educ Ctr, Gainesville, FL 32703 USA 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Xiaojun,Liu, Lina,Li, Ping,et al. A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens[J]. PLANT JOURNAL,2019:10. |
| APA | Pu, Xiaojun.,Liu, Lina.,Li, Ping.,Huo, Heqiang.,Dong, Xiumei.,...&Liu, Li.(2019).A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens.PLANT JOURNAL,10. |
| MLA | Pu, Xiaojun,et al."A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens".PLANT JOURNAL (2019):10. |
入库方式： OAI收割
来源：昆明植物研究所
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