Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
文献类型：期刊论文
| 作者 | Ye, Jun-Wei1,2; Li, De-Zhu1
|
| 刊名 | APPLICATIONS IN PLANT SCIENCES
 |
| 出版日期 | 2019-08-01 |
| 卷号 | 7期号:8页码:4 |
| 关键词 | chloroplast microsatellite Lauraceae Lindera aggregata wuyao |
| ISSN号 | 2168-0450 |
| DOI | 10.1002/aps3.1213 |
| 通讯作者 | Li, De-Zhu(dzl@mail.kib.ac.cn) |
| 英文摘要 | Premise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. Conclusions The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species. |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000482942400003 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/68820]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 通讯作者 | Li, De-Zhu |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.Shanghai Sci & Technol Museum, Shanghai Nat Hist Museum, Nat Hist Res Ctr, Shanghai 200127, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Jun-Wei,Li, De-Zhu. Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(8):4. |
| APA | Ye, Jun-Wei,&Li, De-Zhu.(2019).Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae).APPLICATIONS IN PLANT SCIENCES,7(8),4. |
| MLA | Ye, Jun-Wei,et al."Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)".APPLICATIONS IN PLANT SCIENCES 7.8(2019):4. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。