中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress

文献类型:期刊论文

作者Yang, Yunqiang1,2,3; Pu, Yanan1,2,3; Yin, Xin1,2,3,4; Du, Jiancan2; Zhou, Zhili1,2,3; Yang, Danni1,2,3,4; Sun, Xudong1,2,3; Sun, Hang1,2,3; Yang, Yongping1,2,3
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2019-10-09
卷号67期号:40页码:11077-11088
ISSN号0021-8561
关键词wax esters drought stress alternative splicing turnip BrrWSD1
DOI10.1021/acs.jafc.9b04069
通讯作者Yang, Yongping(yangyp@mail.kib.ac.cn)
英文摘要Cuticular wax accumulation in plants contributes to drought tolerance. Here, we compared the drought levels on two varieties with different genotypes in turnip (Brassica rapa var. rapa) and found that the drought tolerance was higher in the waxy KTRG-B48a than in the wax-free KTRG-B48b. A combination of PacBio and Illumina sequencing analyses revealed that differential transcripts were mainly enriched in the wax synthesis pathway, and a splice variant (BrrWSD1-X2) was identified in the waxy KTRG-B48a. BrrWSD1-X2 had a stronger ability to synthesize wax esters than BrrWSD1-X1 using heterologous expression in yeast (Saccharomyces cerevisiae) mutant H1246a. Then, we speculated that the T to C transversion of the third intron and the higher number of TA repeats in the third intron of BrrWSD1 DNA in the waxy KTRG-B48a may result in a lower efficiency of splicing recognition of the third intron, resulting in the emergence of BrrWSD1-X2 in waxy varieties.
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
语种英语
WOS记录号WOS:000490354700009
源URL[http://ir.kib.ac.cn/handle/151853/69964]  
专题昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Yang, Yongping
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Yang, Yunqiang,Pu, Yanan,Yin, Xin,et al. A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(40):11077-11088.
APA Yang, Yunqiang.,Pu, Yanan.,Yin, Xin.,Du, Jiancan.,Zhou, Zhili.,...&Yang, Yongping.(2019).A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(40),11077-11088.
MLA Yang, Yunqiang,et al."A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.40(2019):11077-11088.

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来源:昆明植物研究所

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