Intervening Effects of Total Alkaloids of Corydalis saxicola Bunting on Rats With Antibiotic-Induced Gut Microbiota Dysbiosis Based on 16S rRNA Gene Sequencing and Untargeted Metabolomics Analyses
文献类型：期刊论文
| 作者 | Liu, Xi1; Zheng, Hua2; Lu, Rigang3; Huang, Huimin1; Zhu, Hongjia1; Yin, Chunli1; Mo, Yiyi1; Wu, Jinxia1; Liu, Xuwen1; Deng, Ming3 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2019-05-31 |
| 卷号 | 10页码:18 |
| ISSN号 | 1664-302X |
| 关键词 | antibiotic-induced gut microbiota dysbiosis 16S rRNA gene sequencing analysis untargeted metabolomics total alkaloids of Corydalis saxicola Bunting (TACS) ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry |
| DOI | 10.3389/fmicb.2019.01151 |
| 英文摘要 | Gut microbiota dysbiosis induced by antibiotics is strongly connected with health concerns. Studying the mechanisms underlying antibiotic-induced gut microbiota dysbiosis could help to identify effective drugs and prevent many serious diseases. In this study, in rats with antibiotic-induced gut microbiota dysbiosis treated with total alkaloids of Corydalis saxicola Bunting (TAGS), urinary and fecal biochemical changes and cecum microbial diversity were investigated using 16S rRNA gene sequencing analysis and untargeted metabolomics. The microbial diversity results showed that 10 genera were disturbed by the antibiotic treatment, and two of them were obviously restored by TAGS. The untargeted metabolomics analysis identified 34 potential biomarkers in urine and feces that may be the metabolites that are most related to the mechanisms underlying antibiotic-induced gut microbiota dysbiosis and the therapeutic effects of TAGS treatment. The biomarkers were involved in six metabolic pathways, comprising pathways related to branched-chain amino acid (BCAA), bile acid, arginine and praline, purine, aromatic amino acid, and amino sugar and nucleotide sugar metabolism. Notably, there was a strong correlation between these metabolic pathways and two gut microbiota genera (g_Blautia and g_Intestinibacter). The correlation analysis suggested that TAGS might synergistically affect four of these metabolic pathways (BCAA, bile acid, arginine and praline, and purine metabolism), thereby modulating gut microbiota dysbiosis. Furthermore, we performed a molecular docking analysis involving simulating high-precision docking and using molecular pathway maps to illuminate the way that ligands (the five main alkaloid components of TAGS) act on a complex molecular network, using CYP27A1 (a key enzyme in the bile acid synthesis pathway) as the target protein. This study provides a comprehensive overview of the intervening effects of TACS on the host metabolic phenotype and gut microbiome in rats with gut microbiota dysbiosis, and it presents new insights for the discovery of effective drugs and the best therapeutic approaches. |
| WOS关键词 | L-LEUCINE SUPPLEMENTATION ; AMINO-ACIDS ; SP NOV. ; RECLASSIFICATION ; HEPATOTOXICITY ; METABONOMICS ; CLOSTRIDIUM ; INTESTINE ; BALANCE ; PLASMA |
| 资助项目 | National Natural Science Foundation of China[81360638] ; National Natural Science Foundation of China[81603533] ; National Natural Science Foundation of China[81560626] ; Guangxi Natural Science Foundation[2012GXNSFBA053108] ; Guangxi Natural Science Foundation[2013GXNSFAA019161] ; program for Scientific Research in Guangxi Higher Education Institutions[200103YB026] ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship[20170703] ; Youth Science Foundation of Guangxi Medical University[GXMUYSF13] ; Youth Science Foundation of Guangxi Medical University[GXMUYSF18] ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China[201410598010] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000470128000001 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Guangxi Natural Science Foundation ; Guangxi Natural Science Foundation ; program for Scientific Research in Guangxi Higher Education Institutions ; program for Scientific Research in Guangxi Higher Education Institutions ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Youth Science Foundation of Guangxi Medical University ; Youth Science Foundation of Guangxi Medical University ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Guangxi Natural Science Foundation ; Guangxi Natural Science Foundation ; program for Scientific Research in Guangxi Higher Education Institutions ; program for Scientific Research in Guangxi Higher Education Institutions ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Youth Science Foundation of Guangxi Medical University ; Youth Science Foundation of Guangxi Medical University ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Guangxi Natural Science Foundation ; Guangxi Natural Science Foundation ; program for Scientific Research in Guangxi Higher Education Institutions ; program for Scientific Research in Guangxi Higher Education Institutions ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Youth Science Foundation of Guangxi Medical University ; Youth Science Foundation of Guangxi Medical University ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Guangxi Natural Science Foundation ; Guangxi Natural Science Foundation ; program for Scientific Research in Guangxi Higher Education Institutions ; program for Scientific Research in Guangxi Higher Education Institutions ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Pharmaceutical Postgraduate Joint Cultivation Base for Innovation & Entrepreneurship ; Youth Science Foundation of Guangxi Medical University ; Youth Science Foundation of Guangxi Medical University ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China ; National Training Programs of Innovation and Entrepreneurship for Undergraduates of China |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/14420]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Guo, Hongwei; Song, Hui; Su, Zhiheng |
| 作者单位 | 1.Guangxi Med Univ, Pharmaceut Coll, Nanning, Peoples R China 2.Guangxi Med Univ, Life Sci Inst, Nanning, Peoples R China 3.Guangxi Inst Food & Drug Control, Nanning, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xi,Zheng, Hua,Lu, Rigang,et al. Intervening Effects of Total Alkaloids of Corydalis saxicola Bunting on Rats With Antibiotic-Induced Gut Microbiota Dysbiosis Based on 16S rRNA Gene Sequencing and Untargeted Metabolomics Analyses[J]. FRONTIERS IN MICROBIOLOGY,2019,10:18. |
| APA | Liu, Xi.,Zheng, Hua.,Lu, Rigang.,Huang, Huimin.,Zhu, Hongjia.,...&Su, Zhiheng.(2019).Intervening Effects of Total Alkaloids of Corydalis saxicola Bunting on Rats With Antibiotic-Induced Gut Microbiota Dysbiosis Based on 16S rRNA Gene Sequencing and Untargeted Metabolomics Analyses.FRONTIERS IN MICROBIOLOGY,10,18. |
| MLA | Liu, Xi,et al."Intervening Effects of Total Alkaloids of Corydalis saxicola Bunting on Rats With Antibiotic-Induced Gut Microbiota Dysbiosis Based on 16S rRNA Gene Sequencing and Untargeted Metabolomics Analyses".FRONTIERS IN MICROBIOLOGY 10(2019):18. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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