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Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing

文献类型：期刊论文

作者Jia, Fan1,3,4; Lv, Pei4; Miao, Huan4; Shi, Xiangwei1; Mei, Hongjun5; Li, Li4; Xu, Xiaoqin4; Tao, Sijue4; Xu, Fuqiang1,2,3,4
刊名FRONTIERS IN NEUROANATOMY
出版日期2019-06-21
卷号13页码:9
关键词PRV Bartha strain retrograde trans-multisynaptic tracer neural circuit PRV531 PRV724
ISSN号1662-5129
DOI10.3389/fnana.2019.00063
英文摘要Mapping the neural circuits facilitates understanding the brain's working mechanism. Pseudorabies virus (PRV; Bartha stain) as a tracer can infect neurons and retrogradely transport in neural circuits. To illuminate the network, tracers expressing reporter genes at a high level are needed. In this study, we optimized the expression level of reporter genes and constructed two new retrograde trans-multisynaptic tracers PRV531 and PRV724, which separately express more robust green and red fluorescent proteins than the existing retrograde tracers PRV152 and PRV614. PRV531 and PRV724 can be used for mapping the neural circuit of the central nervous system (CNS) and the peripheral nervous system (PNS). Overall, our work adds two valuable tracers to the toolbox for mapping neural circuits.
WOS关键词POSTTRANSCRIPTIONAL REGULATORY ELEMENT ; GENE ; NEURONS ; VECTOR ; CONNECTIONS ; TRANSGENES ; CERVIX
资助项目National Natural Science Foundation of China[31830035] ; National Natural Science Foundation of China[91732304] ; National Natural Science Foundation of China[31329001] ; National Basic Research Program (973 Program) of China[2015CB755600] ; Major Research Plan of the National Natural Science Foundation of China[91632303] ; [XDB32030200]
WOS研究方向Anatomy & Morphology ; Neurosciences & Neurology
语种英语
WOS记录号WOS:000472658900001
出版者FRONTIERS MEDIA SA
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China
源URL[http://ir.wipm.ac.cn/handle/112942/14494]  
专题中国科学院武汉物理与数学研究所
通讯作者Jia, Fan; Xu, Fuqiang
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China
3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan Inst Phys & Math, Wuhan, Hubei, Peoples R China
4.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, Wuhan, Hubei, Peoples R China
5.Fifth Hosp Wuhan, Dept Orthopaed, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Jia, Fan,Lv, Pei,Miao, Huan,et al. Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing[J]. FRONTIERS IN NEUROANATOMY,2019,13:9.
APA Jia, Fan.,Lv, Pei.,Miao, Huan.,Shi, Xiangwei.,Mei, Hongjun.,...&Xu, Fuqiang.(2019).Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing.FRONTIERS IN NEUROANATOMY,13,9.
MLA Jia, Fan,et al."Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing".FRONTIERS IN NEUROANATOMY 13(2019):9.

入库方式： OAI收割

来源：武汉物理与数学研究所

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