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Detection of neural connections with ex vivo MRI using a ferritin-encoding trans-synaptic virus

文献类型：期刊论文

作者Zheng, Ning3,4; Su, Peng5; Liu, Yue3; Wang, Huadong3; Nie, Binbin1,4; Fang, Xiaohui3; Xu, Yue3; Lin, Kunzhang3; Lv, Pei3; He, Xiaobin3
刊名NEUROIMAGE
出版日期2019-08-15
卷号197页码:133-142
ISSN号1053-8119
关键词Ferritin Synaptically connected neural networks MRI Fluorescent imaging VSV
DOI10.1016/j.neuroimage.2019.04.039
英文摘要The elucidation of neural networks is essential to understanding the mechanisms of brain functions and brain disorders. Neurotropic virus-based trans-synaptic tracing tools have become an effective method for dissecting the structure and analyzing the function of neural-circuitry. However, these tracing systems rely on fluorescent signals, making it hard to visualize the panorama of the labeled networks in mammalian brain in vivo. One MRI method, Diffusion Tensor Imaging (DTI), is capable of imaging the networks of the whole brain in live animals but without information of anatomical connections through synapses. In this report, a chimeric gene coding for ferritin and enhanced green fluorescent protein (EGFP) was integrated into Vesicular stomatitis virus (VSV), a neurotropic virus that is able to spread anterogradely in synaptically connected networks. After the animal was injected with the recombinant VSV (rVSV), rVSV-Ferritin-EGFP, into the somatosensory cortex (SC) for four days, the labeled neural-network was visualized in the postmortem whole brain with a T2-weighted MRI sequence. The modified virus transmitted from SC to synaptically connected downstream regions. The results demonstrate that rVSV-Ferritin-EGFP could be used as a bimodal imaging vector for detecting synaptically connected neural-network with both ex vivo MRI and fluorescent imaging. The strategy in the current study has the potential to longitudinally monitor the global structure of a given neural-network in living animals.
WOS关键词GENE-EXPRESSION ; NERVOUS-SYSTEM ; REPORTER GENE ; BRAIN ; NEURONS ; CONTRAST ; CIRCUITS ; VECTORS ; ANTEROGRADE ; SUFFICIENT
资助项目Chinese Ministry of Science and Technology[2015CB755601] ; Chinese Ministry of Science and Technology[2015AA020508] ; Chinese Academy of Science[XDB02050005] ; National Natural Science Foundation of China[31400976] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[Y6Y0021004]
WOS研究方向Neurosciences & Neurology ; Radiology, Nuclear Medicine & Medical Imaging
语种英语
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号WOS:000472161500012
资助机构Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences
源URL[http://ir.wipm.ac.cn/handle/112942/14526]  
专题中国科学院武汉物理与数学研究所
通讯作者Wang, Jie; Xu, Fuqiang
作者单位1.Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Radiat & Nucl Energy Technol, Beijing 100049, Peoples R China
2.Univ West London, Sch Human & Social Sci, London, England
3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China
6.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligent Technol, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Ning,Su, Peng,Liu, Yue,et al. Detection of neural connections with ex vivo MRI using a ferritin-encoding trans-synaptic virus[J]. NEUROIMAGE,2019,197:133-142.
APA Zheng, Ning.,Su, Peng.,Liu, Yue.,Wang, Huadong.,Nie, Binbin.,...&Xu, Fuqiang.(2019).Detection of neural connections with ex vivo MRI using a ferritin-encoding trans-synaptic virus.NEUROIMAGE,197,133-142.
MLA Zheng, Ning,et al."Detection of neural connections with ex vivo MRI using a ferritin-encoding trans-synaptic virus".NEUROIMAGE 197(2019):133-142.

入库方式： OAI收割

来源：武汉物理与数学研究所

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