中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage

文献类型：期刊论文

作者Guan, Zeyuan3,4; Pei, Kai3,4; Wang, Jing3,4; Cui, Yongqing3,4; Zhu, Xiang3,4; Su, Xiang3,4; Zhou, Yuanbao3,4; Zhang, Delin3,4; Tang, Chun1,5; Yin, Ping3,4
刊名CELL DISCOVERY
出版日期2019-05-28
卷号5页码:14
ISSN号2056-5968
DOI10.1038/s41421-019-0101-2
英文摘要A newly identified arbitrium communication system regulates the lysis-to-lysogeny decision in a Bacillus bacteriophage. This system contains an arbitrium hexapeptide as a signal, the cellular receptor AimR, and the lysogenic negative regulator AimX. AimR specifically targets the downstream DNA to activate aimX gene expression. The arbitrium peptide binds to AimR, inhibiting its DNA-binding to promote phage lysogeny. Recently, we and other groups have elucidated how arbitrium peptide sensed by AimR. However, the molecular mechanisms of DNA recognition by AimR and the regulation of its DNA-binding activity by the peptide remain largely unknown. Here, we report the crystal structure of the AimR-DNA complex at 2.1 angstrom resolution. The N-terminal H T H motif recognizes the palindromic DNA sequence, buttressed by interactions between positively charged residues and the DNA phosphate groups. The DNA-bound AimR assembles a more closed dimer than the peptide-bound form. Single-molecule FRET and crosslinking assays revealed that the AimR protein samples both open and closed conformations in solution. Arbitrium peptide binding induces a closed-to-open conformational change of AimR, eliminating DNA targeting. Our structural and functional analysis provides new insights into the DNA recognition mechanism of AimR and its regulation by the arbitrium peptide in the context of phage lysis-lysogeny decisions.
WOS关键词MACROMOLECULAR CRYSTALLOGRAPHY BEAMLINE ; TO-CELL COMMUNICATION ; QUORUM ; MODEL ; PLCR
资助项目National Key R&D Program of China[2018YFA0507700] ; National Natural Science Foundation of China[81601/42] ; Fundamental Research Funds for the Central Universities[2662014BQ028] ; Fundamental Research Funds for the Central Universities[2662017PY031]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000471023100001
出版者NATURE PUBLISHING GROUP
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
源URL[http://ir.wipm.ac.cn/handle/112942/14532]  
专题中国科学院武汉物理与数学研究所
通讯作者Zou, Tingting
作者单位1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China
4.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Hubei, Peoples R China
5.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Guan, Zeyuan,Pei, Kai,Wang, Jing,et al. Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage[J]. CELL DISCOVERY,2019,5:14.
APA Guan, Zeyuan.,Pei, Kai.,Wang, Jing.,Cui, Yongqing.,Zhu, Xiang.,...&Zou, Tingting.(2019).Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage.CELL DISCOVERY,5,14.
MLA Guan, Zeyuan,et al."Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage".CELL DISCOVERY 5(2019):14.

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。