Protein stability analysis in ionic liquids by F-19 NMR
文献类型：期刊论文
| 作者 | Cheng, Kai; Wu, Qiong; Jiang, Ling; Liu, Maili; Li, Conggang |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 411期号:19页码:4929-4935 |
| 关键词 | Ionic liquids Protein stability quantification F-19 NMR |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-019-01804-3 |
| 英文摘要 | Ionic liquids have been extensively used as environmentally friendly solvents for enzymatic reactions and other biological systems. Understanding the mechanism of how ionic liquids affect protein stability is crucial for the biological reaction processes and protein storage using ionic liquids as solvents. Although effects of ionic liquids on protein stability have been studied, equilibrium thermodynamics of protein stability in ionic liquids has not been quantitatively measured. Herein, we utilized F-19 NMR to measure the equilibrium thermodynamics of protein stability in ionic liquid [C-4-mim]Br. Our results show that proteins are significantly destabilized in [C-4-mim]Br ionic liquids. Our results suggest that F-19 NMR provides a simple and effective way to study the thermodynamics of protein stability in ionic liquids. F-19 NMR will be applicable to facilitate the protein-protein interaction study in ionic liquids. |
| WOS关键词 | ANTARCTICA LIPASE-B ; THERMAL-STABILITY ; HOFMEISTER SERIES ; CYTOCHROME-C ; STABILIZATION ; EXTRACTION ; INTERMEDIATE ; DISSOLUTION ; IMPROVEMENT ; HEMOGLOBIN |
| 资助项目 | Ministry of Science and Technology of China[2017YFA0505400] ; Innovation team of Hubei Province grant[2016CFA002] ; National Natural Sciences Foundation of China[21575156] ; K.C. Wong Education Foundation[QYZDJ-SSW-SLH027] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH027] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000474354500058 |
| 出版者 | SPRINGER HEIDELBERG |
| 资助机构 | Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/14636]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Li, Conggang |
| 作者单位 | Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math,Natl Ctr Magnet Resonance, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Kai,Wu, Qiong,Jiang, Ling,et al. Protein stability analysis in ionic liquids by F-19 NMR[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(19):4929-4935. |
| APA | Cheng, Kai,Wu, Qiong,Jiang, Ling,Liu, Maili,&Li, Conggang.(2019).Protein stability analysis in ionic liquids by F-19 NMR.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(19),4929-4935. |
| MLA | Cheng, Kai,et al."Protein stability analysis in ionic liquids by F-19 NMR".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.19(2019):4929-4935. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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