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Structure-guided post-SELEX optimization of an ochratoxin A aptamer

文献类型：期刊论文

作者Xu, Guohua2; Zhao, Jiajing2,3; Liu, Na2,3; Yang, Minghui2; Zhao, Qiang1,3; Li, Conggang2,3; Liu, Maili2,3
刊名NUCLEIC ACIDS RESEARCH
出版日期2019-06-20
卷号47期号:11页码:5963-5972
ISSN号0305-1048
DOI10.1093/nar/gkz336
英文摘要SELEX is the cornerstone for aptamer research with broad applications in biosensors and medicine. To improve the affinity of selected aptamers, we propose a structure-guided post-SELEX approach, an optimizationmethod based on the precise secondary structure of the aptamer-ligand complex. We demonstrate this approach using the Ochratoxin A (OTA) aptamer. Guided by the structure, we designed a new aptamer whose affinity is improved by more than 50-fold. We also determined the high-resolution NMR structure of the new aptamer-OTA complex and elucidated the discriminatory recognition mechanism of one atomic difference between two analogs, OTA and OTB. The aptamer forms an unusual hairpin structure containing an intramolecular triple helix, which is not seen in the previously determined aptamer complex. The pi-pi stacking, the hydrophobic interaction, hydrogen bonds and halogen bonds between OTA and the aptamer contribute to the recognition of OTA, and the halogen bonds play an important role in discriminating between OTA and OTB. Our results demonstrate that the structure-guided post-SELEX approach improves aptamers affinity. An improved OTA biosensor system might be developed using this new strategy.
WOS关键词DNA APTAMER ; XPLOR-NIH ; NMR ; RECOGNITION ; LIGANDS ; BINDING ; FLUORESCENCE ; ASSIGNMENT ; TOXICITY
资助项目Hubei Province[2016CFA002] ; National Natural Sciences Foundation of China[21190041] ; National Natural Sciences Foundation of China[21735007] ; National Natural Sciences Foundation of China[21605157] ; National Natural Sciences Foundation of China[21874149] ; Ministry of Science and Technology of China[2017YFA0505400] ; K.C. Wong Education Foundation ; National Natural Sciences Foundation of China
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000475702000045
出版者OXFORD UNIV PRESS
资助机构Hubei Province ; Hubei Province ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Hubei Province ; Hubei Province ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Hubei Province ; Hubei Province ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Hubei Province ; Hubei Province ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China
源URL[http://ir.wipm.ac.cn/handle/112942/14713]  
专题中国科学院武汉物理与数学研究所
通讯作者Li, Conggang
作者单位1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst,Wuhan Inst Phy, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Natl Lab Op, Wuhan 430071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Guohua,Zhao, Jiajing,Liu, Na,et al. Structure-guided post-SELEX optimization of an ochratoxin A aptamer[J]. NUCLEIC ACIDS RESEARCH,2019,47(11):5963-5972.
APA Xu, Guohua.,Zhao, Jiajing.,Liu, Na.,Yang, Minghui.,Zhao, Qiang.,...&Liu, Maili.(2019).Structure-guided post-SELEX optimization of an ochratoxin A aptamer.NUCLEIC ACIDS RESEARCH,47(11),5963-5972.
MLA Xu, Guohua,et al."Structure-guided post-SELEX optimization of an ochratoxin A aptamer".NUCLEIC ACIDS RESEARCH 47.11(2019):5963-5972.

入库方式： OAI收割

来源：武汉物理与数学研究所

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