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NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats

文献类型：期刊论文

作者Du, Hongying2; Li, Shuang3; Zhang, Yingfeng3,4; Guo, Huiling3; Wu, Liang3; Liu, Huili3,4; Manyande, Anne1; Xu, Fuqiang3,4; Wang, Jie3,4
刊名MOLECULES
出版日期2019-07-02
卷号24期号:14页码:16
关键词blood collection serum NMR metabolomics saphenous vein
DOI10.3390/molecules24142542
英文摘要The composition of body fluids has become one of the most commonly used methods for diagnosing various diseases or monitoring the drug responses, especially in serum/plasma. It is therefore vital for investigators to find an appropriate way to collect blood samples from laboratory animals. This study compared blood samples collected from different sites using the NMR based metabolomics approach. Blood samples were collected from the saphenous vein (awake state), tail vein (awake and anesthetized states after administration of sevoflurane or pentobarbital) and the inferior thoracic vena cava (ITVC, anesthetized state). These approaches from the saphenous and tail veins have the potential to enable the collection of multiple samples, and the approach from ITVC is the best method for the collection of blood for the terminate state. The compositions of small molecules in the serum were determined using the H-1-NMR method, and the data were analyzed with traditional correlation analysis, principle component analysis (PCA) and OPLS-DA methods. The results showed that acute anesthesia significantly influenced the composition of serum in a very short period, such as the significant increase in glucose, and decrease in lactate. This indicates that it is better to obtain blood samples under the awake state. From the perspective of animal welfare and multiple sampling, the current study shows that the saphenous vein and tail vein are the best locations to collect multiple blood samples for a reduced risk of injury in the awake state. Furthermore, it is also suitable for investigating pharmacokinetics and the effects of drug intervention on animals.
WOS关键词CLINICAL-CHEMISTRY ; RESONANCE ; SERUM ; H-1 ; SPECTROSCOPY ; METABONOMICS ; METABOLITES ; PLEXUS ; URINE
资助项目National Natural Science Foundation of China[31772047] ; National Natural Science Foundation of China[31771193] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[Y6Y0021004]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者MDPI
WOS记录号WOS:000482303000025
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences
源URL[http://ir.wipm.ac.cn/handle/112942/14855]  
专题中国科学院武汉物理与数学研究所
通讯作者Du, Hongying; Wang, Jie
作者单位1.Univ West London, Sch Human & Social Sci, Brentford TW89GA, Middx, England
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan 430071, Hubei, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Du, Hongying,Li, Shuang,Zhang, Yingfeng,et al. NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats[J]. MOLECULES,2019,24(14):16.
APA Du, Hongying.,Li, Shuang.,Zhang, Yingfeng.,Guo, Huiling.,Wu, Liang.,...&Wang, Jie.(2019).NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats.MOLECULES,24(14),16.
MLA Du, Hongying,et al."NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats".MOLECULES 24.14(2019):16.

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来源：武汉物理与数学研究所

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