Micromixers and their applications in kinetic analysis of biochemical reactions
文献类型：期刊论文
| 作者 | Liu, Chao1,4,5; Li, Ying2,3; Liu, Bi-Feng1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2019-12-01 |
| 卷号 | 205页码:9 |
| 关键词 | Micromixer Fast mixing Kinetics Protein folding DNA/RNA folding Biomolecular interaction Enzyme catalysis |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2019.120136 |
| 英文摘要 | Investigating the kinetics of biochemical reactions plays vital roles in understanding the reaction mechanism. However, many biochemical reactions occur in the time scale of millisecond or even microsecond, which is out of the time-resolution limit of traditional methods. Microfluidic mixers, also referred to micromixers, have been widely developed to resolve the kinetics of biochemical reactions, due to their short mixing time (down to microsecond scale), low sample consumption (as low as several microliters), capability of mixing high-viscosity fluids (up to 35.25 mPa s) and ease of integration with different detection techniques. In this review, we briefly introduced mixing mechanism in micromixers, and discussed the mixing principle of four main types of micromixers: hydrodynamic focusing micromixer, chaotic convection micromixer, droplet micromixer and acoustic micromixer. We then presented how these micromixers applied in investigating enzymatic constant, resolving the kinetics of biomolecule folding or studying biomolecular interactions. At last we summarized the detection technologies used in micromixers for measuring the kinetics and predicted the potential future development on micromixers. We anticipated that micromixers would attract more attentions and would advance the field of kinetics study of biochemical reactions in future. |
| WOS关键词 | EARLY FOLDING KINETICS ; CONTINUOUS-FLOW MIXER ; MICROFLUIDIC MIXER ; G-QUADRUPLEX ; PHOTOCHEMICAL OXIDATION ; CHEMICAL-REACTIONS ; HIGH-THROUGHPUT ; MIXING TIME ; CHIP ; DYNAMICS |
| 资助项目 | National Natural Science Foundation of China[31700746] ; National Key R&D Program of China[2016YFF0100801] ; CAS Pioneer Hundred Talents Program |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000485856500063 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/14918]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Li, Ying; Liu, Bi-Feng |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Dept Biomed Engn,Syst Biol Theme, Coll Life Sci & Technol,Hubei Bioinformat & Mol I, Key Lab Biomed Photon MOE,Wuhan Natl Lab Optoelec, Wuhan 430074, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math,Wuhan Natl Lab Optoelect, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China 4.Hunan Univ, Aptamer Engn Ctr Hunan Prov, Changsha 410082, Hunan, Peoples R China 5.Hunan Univ, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn,Coll Biol, Changsha 410082, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chao,Li, Ying,Liu, Bi-Feng. Micromixers and their applications in kinetic analysis of biochemical reactions[J]. TALANTA,2019,205:9. |
| APA | Liu, Chao,Li, Ying,&Liu, Bi-Feng.(2019).Micromixers and their applications in kinetic analysis of biochemical reactions.TALANTA,205,9. |
| MLA | Liu, Chao,et al."Micromixers and their applications in kinetic analysis of biochemical reactions".TALANTA 205(2019):9. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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