Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor
文献类型：期刊论文
| 作者 | Guo Xingdong1; Yan Chengsong1; Li Hua1; Huang Wenmao2; Shi Xiaoshan1; Huang Min1; Wang Yingfang1; Pan Weiling1; Cai Mingjun3; Li Lunyi1 |
| 刊名 | Cell Research
 |
| 出版日期 | 2017 |
| 卷号 | 27期号:4页码:505 |
| ISSN号 | 1001-0602 |
| 英文摘要 | T-cell receptor-CD3 complex (TCR) is a versatile signaling machine that can initiate antigen-specific immune responses based on various biochemical changes of CD3 cytoplasmic domains, but the underlying structural basis remains elusive. Here we developed biophysical approaches to study the conformational dynamics of CD3 epsilon cytoplasmic domain (CD3 epsilon(CD)). At the single-molecule level, we found that CD3 epsilon(CD) could have multiple conformational states with different openness of three functional motifs, i.e., ITAM, BRS and PRS. These conformations were generated because different regions of CD3 epsilon(CD) had heterogeneous lipid-binding properties and therefore had heterogeneous dynamics. Live-cell imaging experiments demonstrated that different antigen stimulations could stabilize CD3 epsilon(CD) at different conformations. Lipid-dependent conformational dynamics thus provide structural basis for the versatile signaling property of TCR. |
| 资助项目 | [Chinese Academy of Sciences (Strategic Priority Research Program)] ; [National Natural Science Foundation of China] ; [Ministry of Science and Technoloy of China] ; [China Postdoctoral Science Foundation] |
| 语种 | 英语 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/16217]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 作者单位 | 1.中国科学院 2.南京大学 3.中国科学院长春应用化学研究所 4.理海大学 5.中国科学院武汉物理与数学研究所 6.清华大学 |
| 推荐引用方式 GB/T 7714 | Guo Xingdong,Yan Chengsong,Li Hua,et al. Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor[J]. Cell Research,2017,27(4):505. |
| APA | Guo Xingdong.,Yan Chengsong.,Li Hua.,Huang Wenmao.,Shi Xiaoshan.,...&Xu Chenqi.(2017).Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor.Cell Research,27(4),505. |
| MLA | Guo Xingdong,et al."Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor".Cell Research 27.4(2017):505. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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