rapidandsparselabelingofneuronsbasedonthemutantviruslikeparticleofsemlikiforestvirus
文献类型：期刊论文
| 作者 | Jia Fan1; Zhu Xutao1; Lv Pei1; Hu Liang1; Liu Qing1; Jin Sen2; Xu Fuqiang1 |
| 刊名 | neurosciencebulletin
 |
| 出版日期 | 2019 |
| 卷号 | 35期号:3页码:378 |
| ISSN号 | 1673-7067 |
| 英文摘要 | Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant virus-like particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21 (baby hamster kidney) cells. Collectively, the mutant virus-like particle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo. |
| 资助项目 | [National Natural Science Foundation of China] ; [National Basic Research Development Program (973 Program) of China] ; [Strategic Priority Research Program (B), Chinese Academy of Sciences, China] ; [Major Research Plan of the National Natural Science Foundation of China] |
| 语种 | 英语 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/18056]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 作者单位 | 1.中国科学院武汉物理与数学研究所 2.中国科学院大学 |
| 推荐引用方式 GB/T 7714 | Jia Fan,Zhu Xutao,Lv Pei,et al. rapidandsparselabelingofneuronsbasedonthemutantviruslikeparticleofsemlikiforestvirus[J]. neurosciencebulletin,2019,35(3):378. |
| APA | Jia Fan.,Zhu Xutao.,Lv Pei.,Hu Liang.,Liu Qing.,...&Xu Fuqiang.(2019).rapidandsparselabelingofneuronsbasedonthemutantviruslikeparticleofsemlikiforestvirus.neurosciencebulletin,35(3),378. |
| MLA | Jia Fan,et al."rapidandsparselabelingofneuronsbasedonthemutantviruslikeparticleofsemlikiforestvirus".neurosciencebulletin 35.3(2019):378. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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