visualizingtheensemblestructuresofproteincomplexesusingchemicalcrosslinkingcoupledwithmassspectrometry
文献类型：期刊论文
| 作者 | Gong Zhou1; Ding Yuehe2; Dong Xu1; Liu Na2; Erquan Zhang E2; Dong Mengqiu2; Tang Chun1 |
| 刊名 | biophysicsreports
 |
| 出版日期 | 2015 |
| 卷号 | 1期号:3页码:127 |
| ISSN号 | 2364-3439 |
| 英文摘要 | Chemical cross-linking coupled with mass spectrometry (CXMS) identifies protein residues that are close in space, and has been increasingly used for modeling the structures of protein complexes. Here we show that a single structure is usually sufficient to account for the intermolecular cross-links identified for a stable complex with sub-μmol/L binding affinity. In contrast, we show that the distance between two cross-linked residues in the different subunits of a transient or fleeting complex may exceed the maximum length of the cross-linker used, and the cross-links cannot be fully accounted for with a unique complex structure. We further show that the seemingly incompatible cross-links identified with high confidence arise from alternative modes of protein-protein interactions. By converting the intermolecular crosslinks to ambiguous distance restraints, we established a rigid-body simulated annealing refinement protocol to seek the minimum set of conformers collectively satisfying the CXMS data. Hence we demonstrate that CXMS allows the depiction of the ensemble structures of protein complexes and elucidates the interaction dynamics for transient and fleeting complexes. |
| 语种 | 英语 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/18767]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 作者单位 | 1.中国科学院武汉物理与数学研究所 2.National Institute of Biological Sciences |
| 推荐引用方式 GB/T 7714 | Gong Zhou,Ding Yuehe,Dong Xu,et al. visualizingtheensemblestructuresofproteincomplexesusingchemicalcrosslinkingcoupledwithmassspectrometry[J]. biophysicsreports,2015,1(3):127. |
| APA | Gong Zhou.,Ding Yuehe.,Dong Xu.,Liu Na.,Erquan Zhang E.,...&Tang Chun.(2015).visualizingtheensemblestructuresofproteincomplexesusingchemicalcrosslinkingcoupledwithmassspectrometry.biophysicsreports,1(3),127. |
| MLA | Gong Zhou,et al."visualizingtheensemblestructuresofproteincomplexesusingchemicalcrosslinkingcoupledwithmassspectrometry".biophysicsreports 1.3(2015):127. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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