Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo H-1-MRS and Comparison of Detection Results of Tissue Extracts Obtained by H-1-NMR and UHPCL-MS/MS
文献类型：期刊论文
| 作者 | Chen Wei1,2; Lei He-Hua2; Song Tao2; Zhang Li-Min2; Lei Hao2 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-10-01 |
| 卷号 | 47期号:10页码:1671-1679 |
| ISSN号 | 0253-3820 |
| 关键词 | H-1-magnetic resonance spectroscopy In vivo analysis Ultrahigh performance liquid chromatography tandem mass spectrometry Absolute quantification Internal standard |
| DOI | 10.19756/j.issn.0253.3820.191439 |
| 英文摘要 | In vivo proton magnetic resonance spectroscopy (H-1-MRS) can be used to measure regional concentration of multiple brain metabolites simultaneously and non-invasively. The technique has been widely used in the clinical diagnosis and basic researches on neurological/psychiatry diseases. In this study, the concentrations of five cerebral metabolites, namely N-acetyl aspartate (NAA), glutamate (Glu), glutamine (Gln), taurine (Tau) and glutathione (GSH) in two brain regions (i.e., striatum and medial prefrontal cortex) of 12-months old mice were measured by in vivo H-1-MRS using water signal as the internal standard. The detection results were then compared with those obtained by liquid. state H-1-NMR and ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The results demonstrated that the NAA, Glu and Tau concentrations measured by the three techniques had no statistically significant differences among each other, suggesting that the results of in vivo H-1-MRS analyses were at least as reliably and accurate as those obtained by H-1-NMR and UHPLC-MS/MS. The absolute concentrations of Gln and GSH measured by UHPLC-MS/MS were significantly higher or lower, relative to those measured with magnetic resonance techniques. Such discrepancies might have originated from the systematic errors presented in the UHPLC-MS/MS measurements of Gln and GSH. The results demonstrated the feasibility of combined or complementary use of in vivo H-1-MRS and ex vivo measurements for metabolite profiling in the brain. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MOUSE-BRAIN ; NEUROCHEMICAL PROFILE ; PREFRONTAL CORTEX ; RAT HIPPOCAMPUS ; AMINO-ACIDS ; SPECTROSCOPY ; MODEL ; WATER ; AGE |
| 资助项目 | National Natural Science Foundation of China[21790390] ; National Natural Science Foundation of China[21790392] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | SCIENCE PRESS |
| WOS记录号 | WOS:000489332700023 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/21447]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Lei Hao |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Wei,Lei He-Hua,Song Tao,et al. Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo H-1-MRS and Comparison of Detection Results of Tissue Extracts Obtained by H-1-NMR and UHPCL-MS/MS[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2019,47(10):1671-1679. |
| APA | Chen Wei,Lei He-Hua,Song Tao,Zhang Li-Min,&Lei Hao.(2019).Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo H-1-MRS and Comparison of Detection Results of Tissue Extracts Obtained by H-1-NMR and UHPCL-MS/MS.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,47(10),1671-1679. |
| MLA | Chen Wei,et al."Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo H-1-MRS and Comparison of Detection Results of Tissue Extracts Obtained by H-1-NMR and UHPCL-MS/MS".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 47.10(2019):1671-1679. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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