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Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer

文献类型：期刊论文

作者Su, Peng3,4; Wang, Huadong3; Xia, Jinjin3; Zhong, Xin3; Hu, Liang2; Li, Yingli3; Li, Yanqiu3; Ying, Min3; Xu, Fuqiang1,3,4
刊名JOURNAL OF CHEMICAL NEUROANATOMY
出版日期2019-10-01
卷号100页码:11
ISSN号0891-0618
关键词Herpes simplex virus type 1 Anterograde tracer H129 Axon terminal uptake Retrograde labeling
DOI10.1016/j.jchemneu.2019.101662
英文摘要Herpes simplex virus type 1 H129 strain has been widely used as a useful anterograde neuronal circuit tracing tool. However, whether H129 is a rigorous anterograde tracer and undergoes anterograde-only spreading are questions of significant interest. In the present study, we evaluated the retrograde labeling efficiency of H129 using a TK and ICP34.5 dual deleted H129 recombinant (named as H306) which was replication-deficient in non-dividing postmitotic neurons. The novel tracer was tested in vitro and in vivo for evaluating its invasion properties and tracing capacities. The results demonstrated that H306 could efficiently label the neurons following intracerebral injection. Notably, H306 could also efficiently infect upstream innervating neurons through axon terminal uptake and displayed obvious retrograde labeling phenotype, regardless of 3 days or 10 days of tracing. The data implied that replication-competent, trans-multisynaptic H129 tracing results might be a mixed neural networks from two types of starter cells, because the retrogradely infected neurons would also replicate H129 and spread virus anterogradely through their axon collaterals (ectopic starter sites), as the local infected neurons in the injection site (true starter site). Therefore, the interpretation of the anterogradely tracing neural networks by current H129 tools at longer post-inoculation intervals need to be cautious, and effective modification strategies are needed to avoid or block the axon terminal invasion process of H129, which is important for rigorous anterograde H129 tracer.
WOS关键词HERPES-SIMPLEX-VIRUS ; DIRECTIONAL SPREAD ; AMPLICON VECTORS ; BRAIN-STEM ; TRANSPORT ; CIRCUITS ; PATHWAYS ; NEUROVIRULENCE ; NEURONS ; SINGLE
资助项目National Natural Science Foundation of China[31771198] ; National Natural Science Foundation of China[31400976] ; National Natural Science Foundation of China[91632303] ; National Natural Science Foundation of China[91732304] ; Chinese Ministry of Science and Technology[2015CB755601] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB32030200]
WOS研究方向Biochemistry & Molecular Biology ; Neurosciences & Neurology
语种英语
出版者ELSEVIER
WOS记录号WOS:000486357600012
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Ministry of Science and Technology ; Chinese Ministry of Science and Technology ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences
源URL[http://ir.wipm.ac.cn/handle/112942/21472]  
专题中国科学院武汉物理与数学研究所
通讯作者Wang, Huadong; Xu, Fuqiang
作者单位1.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China
2.Wenzhou Med Univ, Sch Biomed Engn, Sch Ophthalmol & Optometry, Wenzhou 325035, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math,Ctr Brain Sci, Wuhan Ctr Magnet Resonance,Key Lab Magnet Resonan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
4.Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelectron, Wuhan 430074, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Su, Peng,Wang, Huadong,Xia, Jinjin,et al. Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer[J]. JOURNAL OF CHEMICAL NEUROANATOMY,2019,100:11.
APA Su, Peng.,Wang, Huadong.,Xia, Jinjin.,Zhong, Xin.,Hu, Liang.,...&Xu, Fuqiang.(2019).Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer.JOURNAL OF CHEMICAL NEUROANATOMY,100,11.
MLA Su, Peng,et al."Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer".JOURNAL OF CHEMICAL NEUROANATOMY 100(2019):11.

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来源：武汉物理与数学研究所

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