A Mast Cell-Specific Receptor Is Critical for Granuloma Induced by Intrathecal Morphine Infusion
文献类型：期刊论文
| 作者 | Zhang, Tao5; Liu, Rui4,5; Che, Delu5; Pundir, Priyanka4; Wang, Nan4,5; Han, Shengli5; Cao, Jiao5; Lv, Yanni5; Dong, Haiyan3; Fang, Fang2 |
| 刊名 | JOURNAL OF IMMUNOLOGY
 |
| 出版日期 | 2019-10-01 |
| 卷号 | 203期号:7页码:1701-1714 |
| ISSN号 | 0022-1767 |
| DOI | 10.4049/jimmunol.1801423 |
| 英文摘要 | Intrathecal morphine infusion is often applied to treat chronic pain related to cancer and other conditions. However, persistent pain can be caused by nerve compression because of granuloma formation. In this study, a mouse model of morphine-induced granuloma formation by intrathecal catheterization morphine infusion into the atlanto-occipital membrane of the foramen magnum was established in wild-type mice, MrgprB2 mutant (MrgprB2(-/-)) mice, and in mast cell-deficient W-sash c-kit mutant (Kit(W-sh/W-sh)) mice. Heat-related pain after surgery was performed to investigate the antipain effect of morphine. H&E staining and immunofluorescence staining of the spinal cord were applied to analyze the mechanism of granuloma formation. Morphine-induced mast cell degranulation was assessed by measuring the Ca2+ influx and mediator release. Anaphylactoid reactions were measured after s.c. morphine infusion to the paws. Chemokine release by mast cells was determined by Human XL Cytokine Array. Experiments with wild-type, MrgprB2 mutant, and mast cell-deficient W-sash c-kit mutant mice demonstrated that morphine activated mast cells and inflammatory cell aggregation through MrgprB2 in intrathecal infusion sites. The chemokine production of human mast cells demonstrated that granuloma formation is correlated with chemokines release. In addition, morphine activated mouse primary mast cells and de novo chemokine synthesis via the MRGPRX2 in human LAD2 cells. We concluded that granuloma formation during intrathecal morphine infusion was associated with MrgprB2/X2. Reducing MRGPRX2 potentially blocks morphine-induced side effects, including granuloma formation. |
| WOS关键词 | INFLAMMATORY MASSES ; DRUG-DELIVERY ; GENE X2 ; THERAPY ; ACTIVATION ; TRENDS |
| 资助项目 | National Natural Science Foundation of China[81230079] ; National Natural Science Foundation of China[81227802] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| 出版者 | AMER ASSOC IMMUNOLOGISTS |
| WOS记录号 | WOS:000487578500005 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/21488]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Dong, Xinzhong; He, Langchong |
| 作者单位 | 1.Xi An Jiao Tong Univ, Sch Basic Med Sci, Xian 710061, Shaanxi, Peoples R China 2.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 3.Xi An Jiao Tong Univ, Dept Pharm, Affiliated Hosp 1, Xian 710061, Shaanxi, Peoples R China 4.Johns Hopkins Univ, Sch Med, Solomon H Snyder Dept Neurosci, Baltimore, MD 21205 USA 5.Xi An Jiao Tong Univ, Sch Pharm, Yanta West Rd, Xian 710061, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Tao,Liu, Rui,Che, Delu,et al. A Mast Cell-Specific Receptor Is Critical for Granuloma Induced by Intrathecal Morphine Infusion[J]. JOURNAL OF IMMUNOLOGY,2019,203(7):1701-1714. |
| APA | Zhang, Tao.,Liu, Rui.,Che, Delu.,Pundir, Priyanka.,Wang, Nan.,...&He, Langchong.(2019).A Mast Cell-Specific Receptor Is Critical for Granuloma Induced by Intrathecal Morphine Infusion.JOURNAL OF IMMUNOLOGY,203(7),1701-1714. |
| MLA | Zhang, Tao,et al."A Mast Cell-Specific Receptor Is Critical for Granuloma Induced by Intrathecal Morphine Infusion".JOURNAL OF IMMUNOLOGY 203.7(2019):1701-1714. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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