Crystal structure of the periplasmic domain of TssL, a key membrane component of Type VI secretion system
文献类型：期刊论文
| 作者 | Wang, XB; Sun, B; Xu, MX; Qiu, SS; Xu, DQ; Ran, TT; He, JH; Wang, WW |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2018 |
| 卷号 | 120期号:-页码:1474-1479 |
| 关键词 | BACTERIAL TYPE VI ENTEROAGGREGATIVE ESCHERICHIA-COLI OUTER-MEMBRANE CYTOPLASMIC DOMAIN VIBRIO-CHOLERAE PROTEIN PEPTIDOGLYCAN LIPOPROTEIN DISSECTION COMPLEXES |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2018.09.166 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Type VI secretion system (1655), as a macromolecular system, is commonly found in Gram-negative bacteria and responsible for exporting effectors. T6SS consists of 13 core proteins. TssL is a component of the membrane complex and plays a pivotal role in T6SS. Here, we report the crystal structure of the C-terminal periplasmic domain of TssL (TssL(Cter)) from Serratia marcescens FS14. The TssL(Cter) (310-503) contain a five-stranded anti-parallel beta-sheet flanked by five alpha-helices and a short N-terminal helix. Structural comparisons revealed that it belongs to the OmpA-like family with a remarked difference in the conformation of the loop3-5. In OmpA-like family, the corresponding loop is located close to loop2-3, forming a cavity with a small opening together with the longest alpha 5, whereas in TssL(Cter), loop3-5 flipped away from this cavity region. In addition, significant differences in the peptidoglycan (PG) binding site suggest that big conformational change must take place to accomplish the PG binding for TssL(Cter). Furthermore, a long flexible loop between helices alpha 1 and alpha 2 is unique in TssL. TssL would have a big conformational change during the delivery of the Hcp needle and effectors. So we speculate that the long flexible endows TssL the adaptation of its evolutionary new function. (C) 2018 Published by Elsevier B.V. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/30894]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201204, Peoples R China 2.Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Key Lab Microbiol Engn Agr Environm,Minist Agr, Nanjing, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, XB,Sun, B,Xu, MX,et al. Crystal structure of the periplasmic domain of TssL, a key membrane component of Type VI secretion system[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,120(-):1474-1479. |
| APA | Wang, XB.,Sun, B.,Xu, MX.,Qiu, SS.,Xu, DQ.,...&Wang, WW.(2018).Crystal structure of the periplasmic domain of TssL, a key membrane component of Type VI secretion system.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,120(-),1474-1479. |
| MLA | Wang, XB,et al."Crystal structure of the periplasmic domain of TssL, a key membrane component of Type VI secretion system".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 120.-(2018):1474-1479. |
入库方式： OAI收割
来源：上海应用物理研究所
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