Guiding protein delivery into live cells using DNA-programmed membrane fusion
文献类型：期刊论文
| 作者 | Sun, LL; Gao, YJ; Wang, YG; Wei, Q; Shi, JY; Chen, N; Li, D; Fan, CH |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2018 |
| 卷号 | 9期号:27页码:5967-5975 |
| 关键词 | EFFICIENT INTRACELLULAR DELIVERY HYBRIDIZATION CHAIN-REACTION LIPID-ANCHORED DNA DRUG-DELIVERY IN-VITRO LIPOSOMES PEPTIDE RELEASE POLYMER SURFACE |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c8sc00367j |
| 文献子类 | 期刊论文 |
| 英文摘要 | Intracellular delivery of proteins provides a direct means to manipulate cell function and probe the intracellular environment. However, direct cytoplasmic delivery of proteins suffers from limited availability of efficient toolsets, and thus remains challenging in research and therapeutic applications. Natural biological cargo delivery processes, like SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex mediated membrane fusion and other vesicle fusion in live cells, enable targeted delivery with high efficiency. A surrogate of SNARE machinery represents a new direction in intracellular protein delivery. Here, we report a DNA-programmed membrane fusion strategy for guiding the efficient intracellular delivery of proteins into live cells. The inherent programmability of DNA hybridization provides spatiotemporal control of the fusion between protein-encapsulated liposomes and cell membranes, enabling rapid release of proteins directly into the cytoplasm, while still remaining functional due to the bypassing of the endosomal trap. We further demonstrate that delivered exogenous Cytochrome c effectively regulates the cell fate. Hence, this DNA-mediated fusion strategy holds great potential for protein drug delivery, regenerative medicine and gene editing. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/30961]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 3.Univ Jinan, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal Univ Shandong, Jinan 250022, Shandong, Peoples R China 4.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, LL,Gao, YJ,Wang, YG,et al. Guiding protein delivery into live cells using DNA-programmed membrane fusion[J]. CHEMICAL SCIENCE,2018,9(27):5967-5975. |
| APA | Sun, LL.,Gao, YJ.,Wang, YG.,Wei, Q.,Shi, JY.,...&Fan, CH.(2018).Guiding protein delivery into live cells using DNA-programmed membrane fusion.CHEMICAL SCIENCE,9(27),5967-5975. |
| MLA | Sun, LL,et al."Guiding protein delivery into live cells using DNA-programmed membrane fusion".CHEMICAL SCIENCE 9.27(2018):5967-5975. |
入库方式： OAI收割
来源：上海应用物理研究所
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