Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction
文献类型：期刊论文
| 作者 | Zhu, D; Liu, W; Cao, WF; Chao, J; Su, S; Wang, LH; Fan, CH |
| 刊名 | ELECTROANALYSIS
 |
| 出版日期 | 2018 |
| 卷号 | 30期号:7页码:1349-1356 |
| 关键词 | DNA NANOSTRUCTURE REACTION AMPLIFICATION CANCER GRAPHENE NANOPARTICLES EXPRESSION BIOSENSORS PLATFORM CELLS |
| ISSN号 | 1040-0397 |
| DOI | 10.1002/elan.201700696 |
| 文献子类 | 期刊论文 |
| 英文摘要 | In this work, we present a multiple amplified electrochemical method that can detect target microRNA-21 (miRNA-21) with high sensitivity by using hierarchical flower-like gold nanostructures (HFGNs) and gold-enriched enzyme-free amplification. The HFGNs deposited on ITO conducting glass served as the electrode substrate with large specific surface area, which allowed the immobilization of large amounts of capture DNA (DNA-1). Then, the gold-enriched hybridization chain reaction (HCR) for signal amplification was attached onto the electrode through target miRNA-mediated sandwich hybridization. The initial strands (I-DNA) enriched on gold nanoparticles (AuNPs) provided plenty of sites for the triggering of the HCR reaction after the addition of H1 and H2 strands, which provided exponential increase of binding sites for the loading of electrochemical signal molecules [Ru(NH3)(6)](3+). Therefore, multiple signal amplification could be achieved to reach an ultrasensitive detection of miRNA-21. A wide linear dynamic range from 1fM to 1nM and a detection limit of 0.12fM (S/N=3) was reached with good selectivity. The electrochemical assay method in this work may hold a great potential for clinical diagnosis of genetic disease in the future. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31038]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Inst Adv Mat, Jiangsu Key Lab Biosensors, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai 201800, Peoples R China 3.Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Inst Adv Mat, Key Lab Organ Elect & Informat Displays, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, D,Liu, W,Cao, WF,et al. Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction[J]. ELECTROANALYSIS,2018,30(7):1349-1356. |
| APA | Zhu, D.,Liu, W.,Cao, WF.,Chao, J.,Su, S.,...&Fan, CH.(2018).Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction.ELECTROANALYSIS,30(7),1349-1356. |
| MLA | Zhu, D,et al."Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction".ELECTROANALYSIS 30.7(2018):1349-1356. |
入库方式： OAI收割
来源：上海应用物理研究所
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