Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers
文献类型：期刊论文
| 作者 | Sun, ZB; Fan, JD; Li, HY; Liu, HJ; Nam, D; Kim, C; Kim, Y; Han, YB; Zhang, JH; Yao, SK |
| 刊名 | ACS NANO
 |
| 出版日期 | 2018 |
| 卷号 | 12期号:8页码:7509-7518 |
| 关键词 | COHERENT-LIGHT SOURCE PLASMONIC NANOSTRUCTURES ORIGAMI CRYSTALLOGRAPHY PULSES RECONSTRUCTION NANOPARTICLES ORIENTATION INSTRUMENT RESOLUTION |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.8b01838 |
| 文献子类 | 期刊论文 |
| 英文摘要 | It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the "diffraction-before-destruction" concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular partides. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. The simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31042]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Shandong, Peoples R China 2.SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA 3.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 4.Stanford Univ, Dept Phys, Stanford, CA 94305 USA 5.Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China 6.Pohang Univ Sci & Technol, Pohang Accelerator Lab, Pohang 37673, South Korea 7.Pohang Univ Sci & Technol, Dept Phys, Pohang 37673, South Korea 8.European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany 9.Gwangju Inst Sci & Technol, Dept Phys & Photon Sci, Gwangju 61005, South Korea 10.Gwangju Inst Sci & Technol, Sch Mat Sci & Engn, Gwangju 61005, South Korea |
| 推荐引用方式 GB/T 7714 | Sun, ZB,Fan, JD,Li, HY,et al. Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers[J]. ACS NANO,2018,12(8):7509-7518. |
| APA | Sun, ZB.,Fan, JD.,Li, HY.,Liu, HJ.,Nam, D.,...&Jiang, HFD.(2018).Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers.ACS NANO,12(8),7509-7518. |
| MLA | Sun, ZB,et al."Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers".ACS NANO 12.8(2018):7509-7518. |
入库方式： OAI收割
来源：上海应用物理研究所
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