Programming Enzyme-Initiated Autonomous DNAzyme Nanodevices in Living Cells
文献类型：期刊论文
| 作者 | Chen, F; Bai, M; Cao, K; Zhao, Y; Cao, XW; Wei, J; Wu, N; Li, J; Wang, LH; Fan, CH |
| 刊名 | ACS NANO
 |
| 出版日期 | 2017 |
| 卷号 | 11期号:12页码:11908-11914 |
| 关键词 | QUADRATIC DNA MACHINE INTRACELLULAR TELOMERASE SIGNAL AMPLIFICATION 3-WAY JUNCTION ONE-STEP HYBRIDIZATION RNA MICRORNA NANOPARTICLE NANOSYSTEM |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.7b06728 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Molecular nanodevices are computational assemblers that switch defined states upon external stimulation. However, interfacing artificial nanodevices with natural molecular machineries in living cells remains a great challenge. Here, we delineate a generic method for programming assembly of enzyme-initiated DNAzyme nanodevices (DzNanos). Two programs including split assembly of two partzymes and toehold exchange displacement assembly of one intact DNAzyme initiated by telomerase are computed. The intact one obtains higher assembly yield and catalytic performance ascribed to proper conformation folding and active misplaced assembly. By employing MnO2 nanosheets as both DNA carriers and source of Mn2+ as DNAzyme cofactor, we find that this DzNano is well assembled via a series of conformational states in living cells and operates autonomously with sustained cleavage activity. Other enzymes can also induce corresponding DzNano assembly with defined programming modules. These DzNanos not only can monitor enzyme catalysis in situ but also will enable the implementation of cellular stages, behaviors, and pathways for basic science, diagnostic, and therapeutic applications as genetic circuits. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31077]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 2.Xi An Jiao Tong Univ, Key Lab Biomed Informat Engn, Minist Educ, Sch Life Sci & Technol, Xianning West Rd, Xian 710049, Shaanxi, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, F,Bai, M,Cao, K,et al. Programming Enzyme-Initiated Autonomous DNAzyme Nanodevices in Living Cells[J]. ACS NANO,2017,11(12):11908-11914. |
| APA | Chen, F.,Bai, M.,Cao, K.,Zhao, Y.,Cao, XW.,...&Zhao, YX.(2017).Programming Enzyme-Initiated Autonomous DNAzyme Nanodevices in Living Cells.ACS NANO,11(12),11908-11914. |
| MLA | Chen, F,et al."Programming Enzyme-Initiated Autonomous DNAzyme Nanodevices in Living Cells".ACS NANO 11.12(2017):11908-11914. |
入库方式： OAI收割
来源：上海应用物理研究所
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