Programming Niche Accessibility and In Vitro Stemness with Intercellular DNA Reactions
文献类型：期刊论文
| 作者 | Li, XJ; Xie, XD; Ma, ZW; Li, Q; Liu, L; Hu, XJ; Liu, C; Li, B; Wang, H; Chen, N |
| 刊名 | ADVANCED MATERIALS
 |
| 出版日期 | 2018 |
| 卷号 | 30期号:46页码:- |
| 关键词 | SELF-RENEWAL WNT PROTEINS ELASTIC-MODULUS CELLS GROWTH CULTURE DIFFERENTIATION GENERATION PROMOTE ORIGAMI |
| ISSN号 | 0935-9648 |
| DOI | 10.1002/adma.201804861 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Stem cells generally exist in low abundance and tend to lose stemness in the absence of self-renewal signals. While extracellular-matrix-mimicking techniques have been developed to support stem cell proliferation, the lack of niche cells in these synthetic systems often hampers continuous stem cell expansion and maintenance of pluripotency, which are indispensable for regenerative medicine. Here, an intercellular DNA-reaction-programmed ESPN (expansion of stem cells with pairing niches) strategy is developed for 3D culture of mammary stem cells (MaSCs). Boolean logic operations are implemented to confer DNA-programmed mechanical signaling and genetically engineered morphogen signaling by niche cells, resulting in sustained expansion of MaSCs in vitro. The creation of stem cell niches improves the proliferation of pluripotent cells by four times during one-week culture. This method thus provides a novel approach for logical regulation of stemness and proliferation of stem cells for biomedicine. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31078]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Inst Mol Med, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Publ Hlth, Sch Med, Shanghai 200025, Peoples R China 3.Univ Chinese Acad Sci, Inst Nutr & Hlth, Shanghai Inst Biol Sci, Chinese Acad Sci, Shanghai 200031, Peoples R China 4.Univ Chinese Acad Sci, Div Phys Biol, Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Chinese Acad Sci, Shanghai 201800, Peoples R China 5.Univ Chinese Acad Sci, Bioimaging Ctr, Shanghai 201800, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China 7.Guangzhou Med Univ, Sch Publ Hlth, Guangzhou 511436, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, XJ,Xie, XD,Ma, ZW,et al. Programming Niche Accessibility and In Vitro Stemness with Intercellular DNA Reactions[J]. ADVANCED MATERIALS,2018,30(46):-. |
| APA | Li, XJ.,Xie, XD.,Ma, ZW.,Li, Q.,Liu, L.,...&Song, HY.(2018).Programming Niche Accessibility and In Vitro Stemness with Intercellular DNA Reactions.ADVANCED MATERIALS,30(46),-. |
| MLA | Li, XJ,et al."Programming Niche Accessibility and In Vitro Stemness with Intercellular DNA Reactions".ADVANCED MATERIALS 30.46(2018):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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