Real-time label-free analysis of the thermostability of DNA structures using GelRed
文献类型：期刊论文
| 作者 | Hao, YY; Liu, L; Zhang, LH; Huang, QL; Wang, F; Li, J; Xu, JQ; Wang, LH |
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2018 |
| 卷号 | 29期号:10页码:- |
| 关键词 | RESONANCE ENERGY-TRANSFER IRIDIUM(III) COMPLEX CELLS PROBE STAIN NANOSTRUCTURES METHYLATION DYNAMICS BINDING HYBRID |
| ISSN号 | 1001-8042 |
| DOI | 10.1007/s41365-018-0486-x |
| 文献子类 | 期刊论文 |
| 英文摘要 | In biological systems, conformational transformations of nucleic acids play critical roles in genetic regulation. However, it remains a tricky task to design and optimize specific labeling strategies to track these changes. In this study, we exploited an intercalating fluorescent dye, GelRed, to characterize different DNA structures. We studied the correlation between fluorescence intensity and DNA structural properties. We showed that single-stranded DNAs with predicted self-folded secondary structures show much stronger fluorescence than those without such structures. For double-stranded DNAs, we observed that fluorescence intensity is positively correlated to their GC content. We also demonstrated that GelRed can be used to monitor DNA conformational changes upon temperature variations in real time. Based on these findings, we concluded that the fluorescence intensity of a GelRed-stained DNA structure has a good correlation with its thermostability in the form of a change in Gibbs free energy. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31086]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Univ, Coll Sci, Dept Chem, NEST Lab, Shanghai 200444, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Sci, Div Phys Biol & Bioimaging Ctr, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, YY,Liu, L,Zhang, LH,et al. Real-time label-free analysis of the thermostability of DNA structures using GelRed[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29(10):-. |
| APA | Hao, YY.,Liu, L.,Zhang, LH.,Huang, QL.,Wang, F.,...&Wang, LH.(2018).Real-time label-free analysis of the thermostability of DNA structures using GelRed.NUCLEAR SCIENCE AND TECHNIQUES,29(10),-. |
| MLA | Hao, YY,et al."Real-time label-free analysis of the thermostability of DNA structures using GelRed".NUCLEAR SCIENCE AND TECHNIQUES 29.10(2018):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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