Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging
文献类型：期刊论文
| 作者 | Deng, SH; Xiao, YP; Hu, J; Chen, JF; Wang, YH; Liu, MP |
| 刊名 | CHINESE OPTICS LETTERS
 |
| 出版日期 | 2018 |
| 卷号 | 16期号:11页码:- |
| 关键词 | RESOLUTION ENHANCEMENT DIFFRACTION PROPAGATION MEDIA |
| ISSN号 | 1671-7694 |
| DOI | 10.3788/COL201816.111801 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy( LSFM) with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31102]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Nanchang Univ, Sch Informat Engn, Nanchang 330031, Jiangxi, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, SH,Xiao, YP,Hu, J,et al. Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging[J]. CHINESE OPTICS LETTERS,2018,16(11):-. |
| APA | Deng, SH,Xiao, YP,Hu, J,Chen, JF,Wang, YH,&Liu, MP.(2018).Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging.CHINESE OPTICS LETTERS,16(11),-. |
| MLA | Deng, SH,et al."Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging".CHINESE OPTICS LETTERS 16.11(2018):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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