Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells
文献类型：期刊论文
| 作者 | Zhuang, JL; Wu, YJ; Chen, L; Liang, SP; Wu, MH; Zhou, LD; Fan, CH; Zhang, YQ |
| 刊名 | ADVANCED SCIENCE
 |
| 出版日期 | 2018 |
| 卷号 | 5期号:12页码:- |
| 关键词 | CIRCULATING TUMOR-CELLS RNA-SEQ MIGRATION MOTILITY HETEROGENEITY PLATFORM ZC3H12A |
| ISSN号 | 2198-3844 |
| DOI | 10.1002/advs.201801158 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Efforts have been taken to enhance the study of single-cells, however, the task remains challenging because most previous investigations cannot exclude the interactions between single cells or separately retrieved cells with specificity for further analyses. Here, a single-cell mobility analysis platform (SCM-Chip) is developed that can not only real-time monitor single-cell migration in independent niches but can also selectively recover target cells one by one. The design of each channel with a single-cell capture unit and an outlet enables the system to place single cells in different isolated niches with fluidic capture and to respectively collect target cells based on mobilities. SCM-Chip characterization of breast cancer cells reveals the presence of high- and low-migratory populations. Whole-cell transcriptome analysis establishes that monocyte chemotactic protein induced protein 1 (MCPIP1) is related with cell mobility; cells with a high expression of MCPIP1 exhibit low mobility in vitro and metastasis in vivo. The SCM platform provides a generic tool for accurate single-cell isolation and differentiation that can be readily adapted for the study of cancer and drug development. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31109]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Immunol, 74 Zhongshan 2nd Rd, Guangzhou 510080, Guangdong, Peoples R China 2.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China 4.Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha 410008, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuang, JL,Wu, YJ,Chen, L,et al. Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells[J]. ADVANCED SCIENCE,2018,5(12):-. |
| APA | Zhuang, JL.,Wu, YJ.,Chen, L.,Liang, SP.,Wu, MH.,...&Zhang, YQ.(2018).Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells.ADVANCED SCIENCE,5(12),-. |
| MLA | Zhuang, JL,et al."Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells".ADVANCED SCIENCE 5.12(2018):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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