Ultrasensitive Simultaneous Detection of Multiplex Disease-Related Nucleic Acids Using Double-Enhanced Surface-Enhanced Raman Scattering Nanosensors
文献类型：期刊论文
| 作者 | Guo, RY; Yin, FF; Sun, YD; Mi, L; Shi, L; Tian, ZJ; Li, T |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2018 |
| 卷号 | 10期号:30页码:25770-25778 |
| 关键词 | SIGNAL AMPLIFICATION GOLD NANOPARTICLES SERS DETECTION LABEL-FREE DNA CANCER PROBES NANOSTRUCTURES SENSITIVITY NANOPROBES |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.8b06757 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Developing ultrasensitive probes holds great significance for simultaneous detection of multiplexed cancer-associated nucleic acids. Bimetallic nanoparticles containing silver may be exploited as nanoprobes for disease detection, which can produce stable and strong surface-enhanced Raman scattering (SERS) signals. However, it remains extremely challenging that such SERS nanoprobes are directly synthesized. Herein gold-silver nanosnowmen, grown via a DNA-mediated approach and attached to thiol-containing Raman dyes, are successfully synthesized. Stable SERS-enhanced gold substrates are also prepared and used as the enriching containers, where the capture DNAs are tethered to sense the target genes jointly enhanced by the SERS nanoprobes in a sandwich hybridization assay. This means detection of the target gene can obtain a limit of detection close to 0.839 fM. Such double-enhanced SERS nanosensors are further employed to simultaneously detect the three types of prostate carcinoma-related genes with high sensitivity and specificity, which meanwhile exhibit robust capacity of resisting disturbance in practical samples. Simultaneous and multiplexed detection of cancer-related genes may provide further biomedical applications with new opportunity. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31161]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, RY,Yin, FF,Sun, YD,et al. Ultrasensitive Simultaneous Detection of Multiplex Disease-Related Nucleic Acids Using Double-Enhanced Surface-Enhanced Raman Scattering Nanosensors[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(30):25770-25778. |
| APA | Guo, RY.,Yin, FF.,Sun, YD.,Mi, L.,Shi, L.,...&Li, T.(2018).Ultrasensitive Simultaneous Detection of Multiplex Disease-Related Nucleic Acids Using Double-Enhanced Surface-Enhanced Raman Scattering Nanosensors.ACS APPLIED MATERIALS & INTERFACES,10(30),25770-25778. |
| MLA | Guo, RY,et al."Ultrasensitive Simultaneous Detection of Multiplex Disease-Related Nucleic Acids Using Double-Enhanced Surface-Enhanced Raman Scattering Nanosensors".ACS APPLIED MATERIALS & INTERFACES 10.30(2018):25770-25778. |
入库方式： OAI收割
来源：上海应用物理研究所
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