Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment
文献类型：期刊论文
| 作者 | Gao, YJ; Liu, XG; Sun, LL; Xu, Y; Yang, SC; Fan, CH; Li, D |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY LETTERS
 |
| 出版日期 | 2019 |
| 卷号 | 10期号:1页码:82—89 |
| 关键词 | HORSERADISH-PEROXIDASE TIME KINETICS PROTEINS DYNAMICS OXIDASE VOLUME |
| ISSN号 | 1948-7185 |
| DOI | 10.1021/acs.jpclett.8b03736 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Most proteins perform functions in intracellular milieu. The crowding, compartmentalized cytosol environment affects the protein structure, folding, conformational stability, substrate diffusion, and substrate-enzyme binding. Moreover, enzymes are available at single or very low copy numbers in a cell, and thus the conformation fluctuations of a single enzyme in a crowding environment could also greatly influence its kinetics. However, the crowding effect is poorly understood in the kinetical aspect of enzymatic reactions. In the present study, individual horseradish peroxidase (HRP) is encapsulated in a liposome containing crowding reagents as mimics of viscous cytosol. The confined crowding environment possesses a profound influence on both the catalytic activity and the product inhibition of enzymes. By analyzing the correlation between product generation and product inhibition, we find that the allosteric noncompetitive inhibition of HRP is alleviated in the crowded and confined milieu. Small-angle X-ray scattering experiments provide straightforward proofs of structural changes of enzymes in crowding environments, which are responsible for the reduced enzyme activity and increased enzyme-substrate affinity. We expect that this work may deepen the understanding of correlations between enzymatic conformations and activity performance in real cellular environments. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31462]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Div Phys Biol, Shanghai 201800, Peoples R China; 4.Natl Engn Res Ctr Nanotechnol, Shanghai 200241, Peoples R China 5.Case Western Reserve Univ, Dept Nutr, Cleveland, OH 44106 USA; 6.Case Western Reserve Univ, Ctr Prote, Cleveland, OH 44106 USA; 7.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Bioimaging Ctr, Shanghai 201800, Peoples R China; 8.Shanghai Jiao Tong Univ, Inst Mol Med, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China; 9.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Gao, YJ,Liu, XG,Sun, LL,et al. Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2019,10(1):82—89. |
| APA | Gao, YJ.,Liu, XG.,Sun, LL.,Xu, Y.,Yang, SC.,...&Li, D.(2019).Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,10(1),82—89. |
| MLA | Gao, YJ,et al."Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 10.1(2019):82—89. |
入库方式： OAI收割
来源：上海应用物理研究所
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