Constructing Submonolayer DNA Origami Scaffold on Gold Electrode for Wiring of Redox Enzymatic Cascade Pathways
文献类型：期刊论文
| 作者 | Ge, ZL; Fu, JL; Liu, MH; Jiang, SX; Andreoni, A; Zuo, XL; Liu, Y; Yan, H; Fan, CH |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2019 |
| 卷号 | 11期号:15页码:13881—13887 |
| 关键词 | NUCLEIC-ACIDS NANOSTRUCTURES AMPLIFICATION MONOLAYERS PROXIMITY ASSAY DRUG |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.8b12374 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Advances in biomimetic microelectronics offer a range of patterned assemblies of proteins and cells for in vitro metabolic engineering where coordinated biochemical pathways allow cell metabolism to be characterized and potentially controlled on a chip. To achieve these goals, developing new methods for interfacing biological systems to microelectronic devices has been in urgent demand. Here, we report the assembly of a DNA origami-templated enzymatic cascade (glucose oxidase and horseradish peroxidase) on gold electrodes, where a monolayer of DNA origami is anchored on gold electrodes via Au S chemistry, to create programmable, electrochemically driven biomimetic device containing both biochemical and electronic components. Upon the posing of a specific electrical potential, substrates/products flow through the enzyme pair and the end product transfers electrons to the electrode. The steady state flux of the distance-dependent enzymatic cascade reactions is translated into a steady state current signal that records the overall enzyme activity. This biological system can be finely tuned by varying the distance between the enzyme pair, which opens new routes to interface microelectronic devices to biological functions. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31525]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai 201800, Peoples R China 2.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China; 3.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China; 4.Arizona State Univ, Dept Chem & Biochem, Biodesign Inst, Ctr Mol Design & Biomimet, Tempe, AZ 85287 USA; 5.Rutgers Univ Camden, Dept Chem, Camden, NJ 08102 USA; 6.Rutgers Univ Camden, Ctr Computat & Integrat Biol, Camden, NJ 08102 USA; 7.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ge, ZL,Fu, JL,Liu, MH,et al. Constructing Submonolayer DNA Origami Scaffold on Gold Electrode for Wiring of Redox Enzymatic Cascade Pathways[J]. ACS APPLIED MATERIALS & INTERFACES,2019,11(15):13881—13887. |
| APA | Ge, ZL.,Fu, JL.,Liu, MH.,Jiang, SX.,Andreoni, A.,...&Fan, CH.(2019).Constructing Submonolayer DNA Origami Scaffold on Gold Electrode for Wiring of Redox Enzymatic Cascade Pathways.ACS APPLIED MATERIALS & INTERFACES,11(15),13881—13887. |
| MLA | Ge, ZL,et al."Constructing Submonolayer DNA Origami Scaffold on Gold Electrode for Wiring of Redox Enzymatic Cascade Pathways".ACS APPLIED MATERIALS & INTERFACES 11.15(2019):13881—13887. |
入库方式： OAI收割
来源：上海应用物理研究所
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