Directing curli polymerization with DNA origami nucleators
文献类型：期刊论文
| 作者 | Mao, XH; Li, K; Liu, MM; Wang, XY; Zhao, TX; An, BL; Cui, MK; Li, YF; Pu, JH; Li, J |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2019 |
| 卷号 | 10期号:-页码:— |
| 关键词 | AMYLOID FIBRILS PROTEIN CSGB KINETICS GROWTH |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-019-09369-6 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and then accelerates fibril formation by increasing the proliferation rate of primary pathways. Fibrils grow either out from (departure mode) or towards the nucleators (arrival mode), implying two distinct roles of CsgB: as nucleation sites and as trap sites to capture growing nanofibrils in vicinity. Curli polymerization follows typical stop-and-go dynamics but exhibits a higher instantaneous elongation rate compared with independent fibril growth. This origami nucleator thus provides an in vitro platform for mechanistically probing molecular nucleation and controlling directional fibril polymerization for bionanotechnology. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31562]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Renji Hosp, Sch Chem & Chem Engn, Sch Med, Shanghai 200240, Peoples R China; 2.MIT, Synthet Biol Grp, Res Lab Elect, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 3.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, 500 Dongchuan Rd, Shanghai 200241, Peoples R China; 4.Chinese Acad Sci, Bioimaging Ctr, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; 5.Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med, Shanghai 200240, Peoples R China; 6.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200127, Peoples R China 7.ShanghaiTech Univ, Sch Phys Sci & Technol, Mat & Phys Biol Div, Shanghai 201210, Peoples R China; 8.Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Mao, XH,Li, K,Liu, MM,et al. Directing curli polymerization with DNA origami nucleators[J]. NATURE COMMUNICATIONS,2019,10(-):—. |
| APA | Mao, XH.,Li, K.,Liu, MM.,Wang, XY.,Zhao, TX.,...&Zhong, C.(2019).Directing curli polymerization with DNA origami nucleators.NATURE COMMUNICATIONS,10(-),—. |
| MLA | Mao, XH,et al."Directing curli polymerization with DNA origami nucleators".NATURE COMMUNICATIONS 10.-(2019):—. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。