DNA nanostructures coordinate gene silencing in mature plants
文献类型：期刊论文
| 作者 | Zhang, H; Demirer, GS; Zhang, HL; Ye, TZ; Goh, NS; Aditham, AJ; Cunningham, FJ; Fan, CH; Landry, MP |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2019 |
| 卷号 | 116期号:15页码:7543—7548 |
| 关键词 | CARBON NANOTUBES CELLULAR UPTAKE DELIVERY BIOLOGY NANOPARTICLES PATHWAYS |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1818290116 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Delivery of biomolecules to plants relies on Agrobacterium infection or biolistic particle delivery, the former of which is amenable only to DNA delivery. The difficulty in delivering functional biomolecules such as RNA to plant cells is due to the plant cell wall, which is absent in mammalian cells and poses the dominant physical barrier to biomolecule delivery in plants. DNA nanostructure-mediated biomolecule delivery is an effective strategy to deliver cargoes across the lipid bilayer of mammalian cells; however, nanoparticle-mediated delivery without external mechanical aid remains unexplored for biomolecule delivery across the cell wall in plants. Herein, we report a systematic assessment of different DNA nanostructures for their ability to internalize into cells of mature plants, deliver siRNAs, and effectively silence a constitutively expressed gene in Nicotiana benthamiana leaves. We show that nanostructure internalization into plant cells and corresponding gene silencing efficiency depends on the DNA nanostructure size, shape, compactness, stiffness, and location of the siRNA attachment locus on the nanostructure. We further confirm that the internalization efficiency of DNA nanostructures correlates with their respective gene silencing efficiencies but that the endogenous gene silencing pathway depends on the siRNA attachment locus. Our work establishes the feasibility of biomolecule delivery to plants with DNA nanostructures and both details the design parameters of importance for plant cell internalization and also assesses the impact of DNA nanostructure geometry for gene silencing mechanisms. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31564]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China; 4.Innovat Genom Inst, Berkeley, CA 94720 USA; 5.Univ Calif Berkeley, Calif Inst Quantitat Biosci QB3, Berkeley, CA 94720 USA; 6.Chan Zuckerberg Biohub, San Francisco, CA 94158 USA 7.Univ Calif Berkeley, Dept Chem & Biomol Engn, Berkeley, CA 94720 USA; 8.Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA; |
| 推荐引用方式 GB/T 7714 | Zhang, H,Demirer, GS,Zhang, HL,et al. DNA nanostructures coordinate gene silencing in mature plants[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(15):7543—7548. |
| APA | Zhang, H.,Demirer, GS.,Zhang, HL.,Ye, TZ.,Goh, NS.,...&Landry, MP.(2019).DNA nanostructures coordinate gene silencing in mature plants.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(15),7543—7548. |
| MLA | Zhang, H,et al."DNA nanostructures coordinate gene silencing in mature plants".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.15(2019):7543—7548. |
入库方式： OAI收割
来源：上海应用物理研究所
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