Live-cell imaging of octaarginine-modified polymer dots via single particle tracking
文献类型：期刊论文
| 作者 | Luo, Y; Han, YP; Hu, XJ; Yin, M; Wu, CF; Li, Q; Chen, N; Zhao, Y |
| 刊名 | CELL PROLIFERATION
 |
| 出版日期 | 2019 |
| 卷号 | 52期号:2页码:— |
| 关键词 | QUANTUM DOTS INTRACELLULAR-TRANSPORT PENETRATING PEPTIDES DNA NANOSTRUCTURES SIRNA DELIVERY IN-VITRO NANOPARTICLES ENDOCYTOSIS AUTOPHAGY CYTOTOXICITY |
| ISSN号 | 0960-7722 |
| DOI | 10.1111/cpr.12556 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Objectives Nanocarriers can greatly enhance the cellular uptake of therapeutic agents to regulate cell proliferation and metabolism. Nevertheless, further application of nanocarriers is often limited by insufficient understanding of the mechanisms of their uptake and intracellular behaviour. Materials and methods Fluorescent polymer dots (Pdots) are coated with synthetic octaarginine peptides (R8) and are analysed for cellular uptake and intracellular transportation in HeLa cervical cancer cells via single particle tracking. Results Surface modification with the R8 peptide efficiently improves both cellular uptake and endosomal escape of Pdots. With single particle tracking, we capture the dynamic process of internalization and intracellular trafficking of R8-Pdots, providing new insights into the mechanism of R8 in facilitating nanostructure-based cellular delivery. Furthermore, our results reveal R8-Pdots as a novel type of autophagy inducer. Conclusions This study provides new insights into R8-mediated cellular uptake and endosomal escape of nanocarriers. It potentiates biological applications of Pdots in targeted cell imaging, drug delivery and gene regulation. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31691]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Sichuan Univ, Coll Life Sci, Chengdu, Sichuan, Peoples R China; 2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Peoples R China 3.Shanghai Normal Univ, Dept Chem, Shanghai, Peoples R China; 4.Guangzhou Med Univ, Sch Publ Hlth, Guangzhou, Guangdong, Peoples R China; 5.Chengdu Med Coll, Dept Anat & Histol & Embryol, Dev & Regenerat Key Lab Sichuan Prov, Chengdu, Sichuan, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Luo, Y,Han, YP,Hu, XJ,et al. Live-cell imaging of octaarginine-modified polymer dots via single particle tracking[J]. CELL PROLIFERATION,2019,52(2):—. |
| APA | Luo, Y.,Han, YP.,Hu, XJ.,Yin, M.,Wu, CF.,...&Zhao, Y.(2019).Live-cell imaging of octaarginine-modified polymer dots via single particle tracking.CELL PROLIFERATION,52(2),—. |
| MLA | Luo, Y,et al."Live-cell imaging of octaarginine-modified polymer dots via single particle tracking".CELL PROLIFERATION 52.2(2019):—. |
入库方式： OAI收割
来源：上海应用物理研究所
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